PDB entry 2hyi

View 2hyi on RCSB PDB site
Description: Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA
Class: hydrolase/RNA binding protein/RNA
Keywords: exon junction, splicing, mRNA processing, translation, DEAD-box ATPase, nonsense mediated decay, hydrolase/RNA binding protein/RNA COMPLEX
Deposited on 2006-08-06, released 2006-08-15
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.212
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Protein mago nashi homolog
    Species: Homo sapiens [TaxId:9606]
    Gene: MAGOH
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2hyia_
  • Chain 'B':
    Compound: RNA-binding protein 8A
    Species: Homo sapiens [TaxId:9606]
    Gene: RBM8A, RBM8
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2hyib_
  • Chain 'C':
    Compound: Probable ATP-dependent RNA helicase DDX48
    Species: Homo sapiens [TaxId:9606]
    Gene: DDX48, EIF4A3, KIAA0111
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38919 (Start-410)
      • cloning artifact (411-412)
    Domains in SCOPe 2.08: d2hyic1, d2hyic2, d2hyic3
  • Chain 'D':
    Compound: Protein CASC3
    Species: Homo sapiens [TaxId:9606]
    Gene: CASC3, MLN51
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: 5'-r(*up*up*up*up*up*u)-3'
  • Chain 'G':
    Compound: Protein mago nashi homolog
    Species: Homo sapiens [TaxId:9606]
    Gene: MAGOH
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2hyig_
  • Chain 'H':
    Compound: RNA-binding protein 8A
    Species: Homo sapiens [TaxId:9606]
    Gene: RBM8A, RBM8
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2hyih_
  • Chain 'I':
    Compound: Probable ATP-dependent RNA helicase DDX48
    Species: Homo sapiens [TaxId:9606]
    Gene: DDX48, EIF4A3, KIAA0111
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38919 (Start-410)
      • cloning artifact (411-412)
    Domains in SCOPe 2.08: d2hyii1, d2hyii2, d2hyii3
  • Chain 'J':
    Compound: Protein CASC3
    Species: Homo sapiens [TaxId:9606]
    Gene: CASC3, MLN51
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: 5'-r(*up*up*up*up*up*u)-3'
  • Heterogens: MG, ANP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2hyiA (A:)
mesdfylryyvghkgkfgheflefefrpdgklryannsnykndvmirkeayvhksvmeel
kriiddseitkeddalwpppdrvgrqeleivigdehisfttskigslidvnqskdpeglr
vfyylvqdlkclvfsliglhfkikpi

    Sequence, based on observed residues (ATOM records): (download) 
    >2hyiA (A:)
sdfylryyvghkgkfgheflefefrpdgklryannsnykndvmirkeayvhksvmeelkr
iiddseitkeddalwpppdrvgrqeleivigdehisfttskigslidvnqskdpeglrvf
yylvqdlkclvfsliglhfkikpi

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2hyiB (B:)
pgpqrsvegwilfvtgvheeateedihdkfaeygeiknihlnldrrtgylkgytlveyet
ykeaqaameglngqdlmgqpisvdwcfvrgp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2hyiC (C:)
mattatmatsgsarkrllkeedmtkvefetseevdvtptfdtmglredllrgiyaygfek
psaiqqraikqiikgrdviaqsqsgtgktatfsisvlqcldiqvretqalilaptrelav
qiqkgllalgdymnvqchaciggtnvgedirkldygqhvvagtpgrvfdmirrrslrtra
ikmlvldeademlnkgfkeqiydvyrylppatqvvlisatlpheilemtnkfmtdpiril
vkrdeltlegikqffvavereewkfdtlcdlydtltitqavifcntkrkvdwltekmrea
nftvssmhgdmpqkeresimkefrsgasrvlistdvwargldvpqvsliinydlpnnrel
yihrigrsgrygrkgvainfvknddirilrdieqyystqidempmnvadlile

    Sequence, based on observed residues (ATOM records): (download) 
    >2hyiC (C:)
dmtkvefetseevdvtptfdtmglredllrgiyaygfekpsaiqqraikqiikgrdviaq
sqsgtgktatfsisvlqcldiqvretqalilaptrelavqiqkgllalgdymnvqchaci
ggtnvgedirkldygqhvvagtpgrvfdmirrrslrtraikmlvldeademlnkgfkeqi
ydvyrylppatqvvlisatlpheilemtnkfmtdpirilvkrdeltlegikqffvavere
ewkfdtlcdlydtltitqavifcntkrkvdwltekmreanftvssmhgdmpqkeresimk
efrsgasrvlistdvwargldvpqvsliinydlpnnrelyihrigrsgrygrkgvainfv
knddirilrdieqyystqidempmnvadlile

  • Chain 'D':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >2hyiG (G:)
mesdfylryyvghkgkfgheflefefrpdgklryannsnykndvmirkeayvhksvmeel
kriiddseitkeddalwpppdrvgrqeleivigdehisfttskigslidvnqskdpeglr
vfyylvqdlkclvfsliglhfkikpi

    Sequence, based on observed residues (ATOM records): (download) 
    >2hyiG (G:)
sdfylryyvghkgkfgheflefefrpdgklryannsnykndvmirkeayvhksvmeelkr
iiddseitkeddalwpppdrvgrqeleivigdehisfttskigslidvnqskdpeglrvf
yylvqdlkclvfsliglhfkikpi

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2hyiH (H:)
pgpqrsvegwilfvtgvheeateedihdkfaeygeiknihlnldrrtgylkgytlveyet
ykeaqaameglngqdlmgqpisvdwcfvrgp

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >2hyiI (I:)
mattatmatsgsarkrllkeedmtkvefetseevdvtptfdtmglredllrgiyaygfek
psaiqqraikqiikgrdviaqsqsgtgktatfsisvlqcldiqvretqalilaptrelav
qiqkgllalgdymnvqchaciggtnvgedirkldygqhvvagtpgrvfdmirrrslrtra
ikmlvldeademlnkgfkeqiydvyrylppatqvvlisatlpheilemtnkfmtdpiril
vkrdeltlegikqffvavereewkfdtlcdlydtltitqavifcntkrkvdwltekmrea
nftvssmhgdmpqkeresimkefrsgasrvlistdvwargldvpqvsliinydlpnnrel
yihrigrsgrygrkgvainfvknddirilrdieqyystqidempmnvadlile

    Sequence, based on observed residues (ATOM records): (download) 
    >2hyiI (I:)
dmtkvefetseevdvtptfdtmglredllrgiyaygfekpsaiqqraikqiikgrdviaq
sqsgtgktatfsisvlqcldiqvretqalilaptrelavqiqkgllalgdymnvqchaci
ggtnvgedirkldygqhvvagtpgrvfdmirrrslrtraikmlvldeademlnkgfkeqi
ydvyrylppatqvvlisatlpheilemtnkfmtdpirilvkrdeltlegikqffvavere
ewkfdtlcdlydtltitqavifcntkrkvdwltekmreanftvssmhgdmpqkeresimk
efrsgasrvlistdvwargldvpqvsliinydlpnnrelyihrigrsgrygrkgvainfv
knddirilrdieqyystqidempmnvadlile

  • Chain 'J':
    No sequence available.

  • Chain 'L':
    No sequence available.