PDB entry 2gcb

View 2gcb on RCSB PDB site
Description: G51S/S52T double mutant of L. casei FPGS
Class: Ligase
Keywords: ATPase, site-directed mutant, P-loop enzyme
Deposited on 2006-03-13, released 2006-06-27
The last revision prior to the SCOP 1.75 freeze date was dated 2006-06-27, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.199
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Folylpolyglutamate synthase
    Species: Lactobacillus casei
    Gene: fgs
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15925 (0-End)
      • engineered (50-51)
      • conflict (164)
    Domains in SCOP 1.75: d2gcba1, d2gcba2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2gcbA (A:)
    mnytetvayihsfprlaktgdhrriltllhalgnpqqqgryihvtgtngkstaanaiahv
    leasgltvglytspfimrfnerimidhepipdaalvnavafvraalerlqqqqadfnvte
    fefitalgywyfrqrqvdvavievgiggdtdstnvitpvvsvltsvaldhqkllghtita
    iakhkagiikrgipvvtgnlvpdaaavvaakvattgsqwlrfdrdfsvpkaklhgwgqrf
    tyedqdgrisdlevplvgdyqqrnmaiaiqtakvyakqtewpltpqnirqglaashwpar
    lekisdtplividgahnpdginglitalkqlfsqpitviagiladkdyaamadrltaafs
    tvylvpvpgtpralpeagyealhegrlkdswqealaaslndvpdqpivitgslylasavr
    qtllggks
    

    Sequence, based on observed residues (ATOM records): (download)
    >2gcbA (A:)
    mnytetvayihsfprlaktgdhrriltllhalgnpqqqgryihvtgtngkstaanaiahv
    leasgltvglytspfimrfnerimidhepipdaalvnavafvraalerlqqqqadfnvte
    fefitalgywyfrqrqvdvavievgiggdtdstnvitpvvsvltsvaldhqkllghtita
    iakhkagiikrgipvvtgnlvpdaaavvaakvattgsqwlrfdrdfsvpkaklhgwgqrf
    tyedqdgrisdlevplvgdyqqrnmaiaiqtakvyakqtewpltpqnirqglaashwpar
    lekisdtplividgahnpdginglitalkqlfsqpitviagilmadrltaafstvylvpv
    pgtpralrlkdswqealaaslndvpdqpivitgslylasavrqtllg