PDB entry 2fmo

View 2fmo on RCSB PDB site
Description: Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase
Class: oxidoreductase
Keywords: oxidoreductase, tim barrel, flavin, reductase
Deposited on 2006-01-09, released 2006-04-25
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.25 Å
R-factor: 0.219
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (176)
    Domains in SCOPe 2.07: d2fmoa_
  • Chain 'B':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (176)
    Domains in SCOPe 2.07: d2fmob_
  • Chain 'C':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (176)
    Domains in SCOPe 2.07: d2fmoc_
  • Heterogens: SO4, FAD, MPD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2fmoA (A:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmoA (A:)
qinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtygadrthsiikgikdrtgleaa
phltcidatpdelrtiardywnngirhivalrgdlemyasdlvtllkevadfdisvaayp
evhpeaksaqadllnlkrkvdagvnraitqfffdvesylrfrdrcvsagidveiipgilp
vsnfkqakkfadmtnvripawmaqmfdgldddaetrklvganiamdmvkilsregvkdfh
fytlnraemsyaichtlgvrp

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2fmoB (B:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmoB (B:)
qslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtygansgerdrthsi
ikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgdlppemyasdlvtl
lkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnraitqfffdvesylrfrdrc
vsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfdgldddaetrklvganiam
dmvkilsregvkdfhfytlnraemsyaichtlgvrp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2fmoC (C:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmoC (C:)
qinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtyganserdrthsiikgikdrtg
leaaphltcidatpdelrtiardywnngirhivalrgdlemyasdlvtllkevadfdisv
aaypevhpeaksaqadllnlkrkvdagvnraitqfffdvesylrfrdrcvsagidveiip
gilpvsnfkqakkfadmtnvripawmaqmfdgldddaetrklvganiamdmvkilsregv
kdfhfytlnraemsyaichtlgvrp