PDB entry 2e3y

View 2e3y on RCSB PDB site
Description: Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A
Class: ligase
Keywords: Biotin Biosynthesis, Dimer, X-ray Diffraction, Structural Genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI, LIGASE
Deposited on 2006-12-01, released 2007-06-05
The last revision prior to the SCOPe 2.08 freeze date was dated 2008-02-05, with a file datestamp of 2008-02-01.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: 0.187
AEROSPACI score: 0.63 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: biotin--[acetyl-CoA-carboxylase] ligase
    Species: Pyrococcus horikoshii
    Gene: bpl
    Database cross-references and differences (RAF-indexed):
    • Uniprot O57883 (0-234)
      • engineered (47)
      • engineered (110)
    Domains in SCOPe 2.08: d2e3ya1, d2e3ya2
  • Chain 'B':
    Compound: biotin--[acetyl-CoA-carboxylase] ligase
    Species: Pyrococcus horikoshii
    Gene: bpl
    Database cross-references and differences (RAF-indexed):
    • Uniprot O57883 (0-234)
      • engineered (47)
      • engineered (110)
    Domains in SCOPe 2.08: d2e3yb1, d2e3yb2
  • Heterogens: ADN, BTN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2e3yA (A:)
mlglktsiigrrviyfqeitstnefaktsyleegtvivadkqtmghgalnrkwespeggl
wlsivlspkvpqkdlpkivflgavgvvetlkefsidgrikwpndvlvnykaiagvlvegk
gdkivlgiglnvnnkvpngatsmklelgsevpllsvfrslitnldrlylnflknpmdiln
lvrdnmilgvrvkilgdgsfegiaediddfgrliirldsgevkkviygdvslrfl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2e3yB (B:)
mlglktsiigrrviyfqeitstnefaktsyleegtvivadkqtmghgalnrkwespeggl
wlsivlspkvpqkdlpkivflgavgvvetlkefsidgrikwpndvlvnykaiagvlvegk
gdkivlgiglnvnnkvpngatsmklelgsevpllsvfrslitnldrlylnflknpmdiln
lvrdnmilgvrvkilgdgsfegiaediddfgrliirldsgevkkviygdvslrfl

    Sequence, based on observed residues (ATOM records): (download) 
    >2e3yB (B:)
mlglktsiigrrviyfqeitstnefaktsyleegtvivadkqtmghgalnkwespegglw
lsivlspkvpqkdlpkivflgavgvvetlkefsidgrikwpndvlvnykaiagvlvegkg
dkivlgiglnvnnkvpngatsmklelgsevpllsvfrslitnldrlylnflknpmdilnl
vrdnmilgvrvkilgdgsfegiaediddfgrliirldsgevkkviygdvslrfl