PDB entry 2e2i

View 2e2i on RCSB PDB site
Description: RNA polymerase II elongation complex in 5 mM Mg+2 with 2'-dGTP
Class: transcription/DNA/RNA
Keywords: transcription, mRNA, multiprotein complex, molecular machine, DNA
Deposited on 2006-11-14, released 2006-12-19
The last revision prior to the SCOP 1.75 freeze date was dated 2006-12-19, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 3.41 Å
R-factor: 0.269
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase II largest subunit
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ia1
  • Chain 'B':
    Compound: DNA-directed RNA polymerase II 140 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ib1
  • Chain 'C':
    Compound: DNA-directed RNA polymerase II 45 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ic1, d2e2ic2
  • Chain 'E':
    Compound: DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ie1, d2e2ie2
  • Chain 'F':
    Compound: DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2if1
  • Chain 'H':
    Compound: DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ih1
  • Chain 'I':
    Compound: DNA-directed RNA polymerase II subunit 9
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ii1, d2e2ii2
  • Chain 'J':
    Compound: DNA-directed RNA polymerases I/II/III subunit 10
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ij1
  • Chain 'K':
    Compound: DNA-directed RNA polymerase II 13.6 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2ik1
  • Chain 'L':
    Compound: DNA-directed RNA polymerases I, II, and III 7.7 kDa polypeptide
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.75: d2e2il1
  • Chain 'N':
    Compound: 5'-d(*cp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*g)-3'
  • Chain 'R':
    Compound: 5'-r(*ap*up*cp*gp*ap*gp*ap*gp*gp*a)-3'
  • Chain 'T':
    Compound: 28-MER DNA template strand
  • Heterogens: ZN, MG, DGT

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2e2iA (A:)
mvgqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgs
idrnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehne
lmrqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglk
lvgswkkdratgdadepelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclp
vppppvrpsisfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhv
atymdndiagqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnle
ldqvgvpksiaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskr
agdiqlqygwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspyna
dfdgdemnlhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtf
ieldqvlnmlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllsp
kdngmliidgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhn
gfstgigdtiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvr
flneardkagrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfv
drtlphfskddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrr
lvkaledimvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvd
llntdhtldpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvni
rriiqnaqqtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfcc
llrsrlatrrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmt
lntfhfagvaskkvtsgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaieh
ttlksvtiaseiyydpdprstvipedeeiiqlhfslldeeaeqsfdqqspwllrleldra
amndkdltmgqvgerikqtfkndlfviwsedndekliircrvvrpksldaeteaeedhml
kkientmlenitlrgveniervvmmkydrkvpsptgeyvkepewvletdgvnlsevmtvp
gidptriytnsfidimevlgieagraalykevynviasdgsyvnyrhmallvdvmttqgg
ltsvtrhgfnrsntgalmrcsfeetveilfeagasaelddcrgvsenvilgqmapigtga
fdvmideeslvkympeqkiteiedgqdggvtpysnesglvnadldvkdelmfsplvdsgs
ndamaggftayggadygeatspfgaygeaptspgfgvsspgfsptsptysptspayspts
psysptspsysptspsysptspsysptspsysptspsysptspsysptspsysptspsys
ptspsysptspsysptspsysptspsysptspsysptspaysptspsysptspsysptsp
sysptspsysptspnysptspsysptspgyspgspayspkqdeqkhnenensr

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iA (A:)
gqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgsid
rnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehnelm
rqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglklv
gswkkdratelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclpvppppvrp
sisfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhvatymdndi
agqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnleldqvgvpk
siaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskragdiqlqy
gwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspynadfdgdemn
lhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtfieldqvln
mlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllspkdngmlii
dgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhngfstgigd
tiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvrflneardk
agrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfvdrtlphfs
kddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrrlvkaledi
mvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvdllntdhtl
dpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvnirriiqnaq
qtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfccllrsrlat
rrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmtlkkvtsgv
prlkeilnvaknmktpsltvylepghaadqeqaklirsaiehttlksvtiaseiyydpdp
rstvipedeeiiqlhfslqqspwllrleldraamndkdltmgqvgerikqtfkndlfviw
sedndekliircrvvaeedhmlkkientmlenitlrgveniervvmmkydrkvpsptgey
vkepewvletdgvnlsevmtvpgidptriytnsfidimevlgieagraalykevynvias
dgsyvnyrhmallvdvmttqggltsvtrhgfnrsntgalmrcsfeetveilfeagasael
ddcrgvsenvilgqmapigtgafdvmidees

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2e2iB (B:)
msdlansekyydedpygfedesapitaedswavisaffrekglvsqqldsfnqfvdytlq
diicedstlileqlaqhttesdnisrkyeisfgkiyvtkpmvnesdgvthalypqearlr
nltyssglfvdvkkrtyeaidvpgrelkyeliaeeseddsesgkvfigrlpimlrskncy
lseatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaei
rsalekgsrfistlqvklygregssartikatlpyikqdipiviifralgiipdgeileh
icydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkef
lphitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktl
fkkltkdifrymqrtveeahdfnmklainaktitsglkyalatgnwgeqkkamssragvs
qvlnrytysstlshlrrtntpigrdgklakprqlhnthwglvcpaetpegqacglvknls
lmscisvgtdpmpiitflsewgmepledyvphqspdatrvfvngvwhgvhrnparlmetl
rtlrrkgdinpevsmirdirekelkiftdagrvyrplfiveddeslghkelkvrkghiak
lmateyqdieggfedveeytwssllneglveyidaeeeesiliamqpedlepaeaneend
ldvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprntyqsamgkqamgv
fltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaiacysgynqedsmi
mnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtydkldddglia
pgvrvsgedviigkttpispdeeelgqrtayhskrdastplrstengivdqvlvttnqdg
lkfvkvrvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsr
mtvahliecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkk
lmaqiffgptyyqrlrhmvddkiharargpmqvltrqpvegrsrdgglrfgemerdcmia
hgaasflkerlmeasdafrvhicgicglmtviaklnhnqfeckgcdnkidiyqihipyaa
kllfqelmamnitprlytdrsrdf

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iB (B:)
desapitaedswavisaffrekglvsqqldsfnqfvdytlqdiicedstlileqyeisfg
kiyvtkpmvnesdgvthalypqearlrnltyssglfvdvkkrtyeaidvpgrelkvfigr
lpimlrskncylseatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkka
apspishvaeirsalekgsrfistlqvklygregssartikatlpyikqdipiviifral
giipdgeilehicydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekri
qyakdilqkeflphitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldla
gpllaqlfktlfkkltkdifrymqrtvenmklainaktitsglkyalatgnwgeqkkams
sragvsqvlnrytysstlshlrrtntpigrdgklakprqlhnthwglvcpaetpegqacg
lvknlslmscisvgtdpmpiitflsewgmepledyvphqspdatrvfvngvwhgvhrnpa
rlmetlrtlrrkgdinpevsmirdirekelkiftdagrvyrplfiveddeslghkelkvr
kghiaklmateyqdveeytwssllneglveyidaeeeesiliamqpedlepaedvdpakr
irvshhattfthceihpsmilgvaasiipfpdhnqsprntyqsamgkqamgvfltnynvr
mdtmanilyypqkplgttrameylkfrelpagqnaivaiacysgynqedsmimnqssidr
glfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtydkldddgliapgvrvsge
dviigkttpisskrdastplrstengivdqvlvttnqdglkfvkvrvrttkipqigdkfa
srhgqkgtigityrredmpftaegivpdliinphaipsrmtvahliecllskvaalsgne
gdaspftditvegiskllrehgyqsrgfevmynghtgkklmaqiffgptyyqrlrhmvdd
kiharargpmqvltrqpvegrsrdgglrfgemerdcmiahgaasflkerlmeasdafrvh
icgicglmtviaklnhnqfeckgcdnkidiyqihipyaakllfqelmamnitprlytdrs
rdf

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2e2iC (C:)
mseegpqvkireaskdnvdfilsnvdlamanslrrvmiaeiptlaidsvevetnttvlad
efiahrlgliplqsmdieqleysrdcfcedhcdkcsvvltlqafgesesttnvyskdlvi
vsnlmgrnighpiiqdkegngvlicklrkgqelkltcvakkgiakehakwgpaaaiefey
dpwnklkhtdywyeqdsakewpqsknceyedppnegdpfdykaqadtfymnvesvgsipv
dqvvvrgidtlqkkvasillaltqmdqdkvnfasgdnntasnmlgsnedvmmtgaeqdpy
snasqmgntgsggydnaw

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iC (C:)
eegpqvkireaskdnvdfilsnvdlamanslrrvmiaeiptlaidsvevetnttvladef
iahrlgliplqsmdieqleysrdcfcedhcdkcsvvltlqafgesesttnvyskdlvivs
nlmgrnighpiiqdkegngvlicklrkgqelkltcvakkgiakehakwgpaaaiefeydp
wnklkhtdywyeqdsakewpqsknceyedppnegdpfdykaqadtfymnvesvgsipvdq
vvvrgidtlqkkvasillaltqmdqd

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2e2iE (E:)
mdqenernisrlwrafrtvkemvkdrgyfitqeevelpledfkakycdsmgrpqrkmmsf
qanpteesiskfpdmgslwvefcdepsvgvktmktfvihiqeknfqtgifvyqnnitpsa
mklvpsippatietfneaalvvnithhelvpkhirlssdekrellkryrlkesqlpriqr
adpvalylglkrgevvkiirksetsgryasyricm

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iE (E:)
qenernisrlwrafrtvkemvkdrgyfitqeevelpledfkakycdsmgrpqrkmmsfqa
npteesiskfpdmgslwvefcdepsvgvktmktfvihiqeknfqtgifvyqnnitpsamk
lvpsippatietfneaalvvnithhelvpkhirlssdekrellkryrlkesqlpriqrad
pvalylglkrgevvkiirksetsgryasyricm

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2e2iF (F:)
msdyeeafndgnenfedfdvehfsdeetyeekpqfkdgettdangktivtggngpedfqq
heqirrktlkekaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplri
amkelaekkiplvirrylpdgsfedwsveelivdl

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iF (F:)
kekaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplriamkelaekk
iplvirrylpdgsfedwsveelivdl

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >2e2iH (H:)
msntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtia
sslnledtpandssatrswrppqagdrsladdydyvmygtaykfeevskdliavyysfgg
llmrlegnyrnlnnlkqenayllirr

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iH (H:)
msntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtia
ssltrswrppqagdrsladdydyvmygtaykfeevskdliavyysfggllmrlegnyrnl
nnlkqenayllirr

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >2e2iI (I:)
mttfrfcrdcnnmlypredkennrllfecrtcsyveeagsplvyrhelitnigetagvvq
digsdptlprsdrecpkchsrenvffqsqqrrkdtsmvlffvclscshiftsdqknkrtq
fs

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iI (I:)
ttfrfcrdcnnmlypredkennrllfecrtcsyveeagsplvyrhelitnigetagvvqd
igsdptlprsdrecpkchsrenvffqsqqrrkdtsmvlffvclscshiftsdqknkrtq

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >2e2iJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynplekrd

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynp

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >2e2iK (K:)
mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtlaaddaf

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iK (K:)
mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtl

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >2e2iL (L:)
msregfqiptnldaaaagtsqartatlkyicaecssklslsrtdavrckdcghrillkar
tkrlvqfear

    Sequence, based on observed residues (ATOM records): (download) 
    >2e2iL (L:)
atlkyicaecssklslsrtdavrckdcghrillkartkrlvqfear

  • Chain 'N':
    No sequence available.

  • Chain 'R':
    No sequence available.

  • Chain 'T':
    No sequence available.