SCOPe 2.08: Structural Classification of Proteins

PDB entry 2d32

View 2d32 on RCSB PDB site
Description: Crystal Structure of Michaelis Complex of gamma-Glutamylcysteine Synthetase
Class: Ligase
Keywords: glutathione homeostasis, peptide synthesis, phosphoryl transfer, Ligase
Deposited on 2005-09-25, released 2006-11-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.17
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Glutamate--cysteine ligase
Species: Escherichia coli [TaxId:562]
Gene: gshI
Database cross-references and differences (RAF-indexed):
- Uniprot P0A6W9 (0-517)
  - engineered (105)
  - engineered (204)
  - engineered (222)
Domains in SCOPe 2.08: d2d32a_
Chain 'B':
Compound: Glutamate--cysteine ligase
Species: Escherichia coli [TaxId:562]
Gene: gshI
Database cross-references and differences (RAF-indexed):
- Uniprot P0A6W9 (0-517)
  - engineered (105)
  - engineered (204)
  - engineered (222)
Domains in SCOPe 2.08: d2d32b_
Chain 'C':
Compound: Glutamate--cysteine ligase
Species: Escherichia coli [TaxId:562]
Gene: gshI
Database cross-references and differences (RAF-indexed):
- Uniprot P0A6W9 (0-End)
  - engineered (105)
  - engineered (204)
Domains in SCOPe 2.08: d2d32c_
Chain 'D':
Compound: Glutamate--cysteine ligase
Species: Escherichia coli [TaxId:562]
Gene: gshI
Database cross-references and differences (RAF-indexed):
- Uniprot P0A6W9 (0-517)
  - engineered (105)
  - engineered (204)
  - engineered (222)
Domains in SCOPe 2.08: d2d32d_
Heterogens: MG, GLU, CYS, ANP, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>2d32A (A:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

Sequence, based on observed residues (ATOM records): (download)

>2d32A (A:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgadakekisagyfrvi
rnyyrfgwvipylfgaspaisssfltslpfektesgmyylpyatslrlsdlgytnksqsn
lgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrv
trsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemsss
elactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggea
yqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedf
vaereaserrqqemeaadtepfavwlekha

Chain 'B':
Sequence, based on SEQRES records: (download)

>2d32B (B:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

Sequence, based on observed residues (ATOM records): (download)

>2d32B (B:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqakkekisagyfrvirnyyr
fgwvipylfgaspaisssfltslpfektesgmyylpyatslrlsdlgytnqsnlgitfnd
lyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrespsd
allrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactrvnw
nrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcdelv
acfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaereaser
rqqemeaaepfavwlekha

Chain 'C':
Sequence, based on SEQRES records: (download)

>2d32C (C:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

Sequence, based on observed residues (ATOM records): (download)

>2d32C (C:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiadielaqygtsn
tgrfktlyreglknrygalmqtisgvhynfslpmafwqakisgadakekisagyfrvirn
yyrfgwvipylfgaspaisssfslpfektmyylpyatslrlsdlgytnqsnlgitfndly
eyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrsgespsd
allrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactrvnw
nrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcdelv
acfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaereaser
rqqemeaadtepfavwlek

Chain 'D':
Sequence, based on SEQRES records: (download)

>2d32D (D:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

Sequence, based on observed residues (ATOM records): (download)

>2d32D (D:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqakakekisagyfrvirnyy
rfgwvipylfgaspaisssfltslpfektesgmyylpyatslrlsdlgytnqsnlgitfn
dlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrsges
psdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactr
vnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcd
elvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaerea
serrqqemeaadtepfavwlekha