PDB entry 2c8n

View 2c8n on RCSB PDB site
Description: the structure of a family 51 arabinofuranosidase, araf51, from clostridium thermocellum in complex with 1,3-linked arabinoside of xylobiose.
Class: hydrolase
Keywords: arabinofuranosidase, glycosidase, mechanism, xylan, arabinan, hydrolase
Deposited on 2005-12-06, released 2005-12-14
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.204
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8na1, d2c8na2
  • Chain 'B':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8nb1, d2c8nb2
  • Chain 'C':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8nc1, d2c8nc2
  • Chain 'D':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8nd1, d2c8nd2
  • Chain 'E':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8ne1, d2c8ne2
  • Chain 'F':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 2C8N
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOPe 2.07: d2c8nf1, d2c8nf2
  • Heterogens: EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2c8nA (A:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nA (A:)
kkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkel
nvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaei
mmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvg
hktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyisl
hqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnn
edanimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapiv
ternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneeke
evtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkss
fddgiltsmlrraswnvirig

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2c8nB (B:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nB (B:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssf
ddgiltsmlrraswnvirig

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2c8nC (C:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nC (C:)
armtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkelnv
piirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeimm
avnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvghk
tmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislhq
yygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnned
animqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivte
rnggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekeev
tifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssfd
dgiltsmlrraswnvirig

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2c8nD (D:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nD (D:)
armtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkelnv
piirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeimm
avnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvghk
tmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislhq
yygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnned
animqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivte
rnggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekeev
tifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssfd
dgitsmlrraswnvirig

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2c8nE (E:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nE (E:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssf
iltsmlrraswnvirig

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2c8nF (F:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c8nF (F:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssf
ddgiltsmlrraswnvirig