PDB entry 2bh2

View 2bh2 on RCSB PDB site
Description: crystal structure of e. coli 5-methyluridine methyltransferase ruma in complex with ribosomal RNA substrate and s-adenosylhomocysteine.
Class: transferase
Keywords: iron-sulfur cluster, methyltransferase, RNA modification, RNA processing, transferase, ruma, base flipping, sam, ob-fold, protein-RNA complex, base stacking, substrate selectivity, general base, product release, 4fe-4s, direct protein sequencing, metal-binding
Deposited on 2005-01-06, released 2005-03-30
The last revision prior to the SCOPe 2.01 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-06.
Experiment type: XRAY
Resolution: 2.15 Å
R-factor: 0.177
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 23s rRNA (uracil-5-)-methyltransferase ruma
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.01: d2bh2a1, d2bh2a2
  • Chain 'B':
    Compound: 23s rRNA (uracil-5-)-methyltransferase ruma
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.01: d2bh2b1, d2bh2b2
  • Chain 'C':
    Compound: 23s ribosomal RNA 1932-1968
    Species: Escherichia coli, synthetic [TaxId:562]
  • Chain 'D':
    Compound: 23s ribosomal RNA 1932-1968
    Species: Escherichia coli, synthetic [TaxId:562]
  • Heterogens: SAH, SF4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2bh2A (A:)
    maqfysakrrtttrqiitvsvndldsfgqgvarhngktlfipgllpqenaevtvtedkkq
    yarakvvrrlsdsperetprcphfgvcggcqqqhasvdlqqrsksaalarlmkhdvsevi
    advpwgyrrrarlslnylpktqqlqmgfrkagssdivdvkqcpilapqleallpkvracl
    gslqamrhlghvelvqatsgtlmilrhtaplssadreklerfshsegldlylapdseile
    tvsgempwydsnglrltfsprdfiqvnagvnqkmvaralewldvqpedrvldlfcgmgnf
    tlplatqaasvvgvegvpalvekgqqnarlnglqnvtfyhenleedvtkqpwakngfdkv
    lldparagaagvmqqiiklepirivyvscnpatlardseallkagytiarlamldmfpht
    ghlesmvlfsrvk
    

    Sequence, based on observed residues (ATOM records): (download)
    >2bh2A (A:)
    qiitvsvndldsfgqgvarhngktlfipgllpqenaevtvtedkkqyarakvvrrlsdsp
    eretprcphfgvcggcqqqhasvdlqqrsksaalarlmkhdvseviadvpwgyrrrarls
    lnylpktqqlqmgfrkagssdivdvkqcpilapqleallpkvraclgslqamrhlghvel
    vqatsgtlmilrhtaplssadreklerfshsegldlylapdseiletvsgempwydsngl
    rltfsprdfiqvnagvnqkmvaralewldvqpedrvldlfcgmgnftlplatqaasvvgv
    egvpalvekgqqnarlnglqnvtfyhenleedvtkqpwakngfdkvlldparagaagvmq
    qiiklepirivyvscnpatlardseallkagytiarlamldmfphtghlesmvlfsrv
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >2bh2B (B:)
    maqfysakrrtttrqiitvsvndldsfgqgvarhngktlfipgllpqenaevtvtedkkq
    yarakvvrrlsdsperetprcphfgvcggcqqqhasvdlqqrsksaalarlmkhdvsevi
    advpwgyrrrarlslnylpktqqlqmgfrkagssdivdvkqcpilapqleallpkvracl
    gslqamrhlghvelvqatsgtlmilrhtaplssadreklerfshsegldlylapdseile
    tvsgempwydsnglrltfsprdfiqvnagvnqkmvaralewldvqpedrvldlfcgmgnf
    tlplatqaasvvgvegvpalvekgqqnarlnglqnvtfyhenleedvtkqpwakngfdkv
    lldparagaagvmqqiiklepirivyvscnpatlardseallkagytiarlamldmfpht
    ghlesmvlfsrvk
    

    Sequence, based on observed residues (ATOM records): (download)
    >2bh2B (B:)
    iitvsvndldsfgqgvarhngktlfipgllpqenaevtvtedkkqyarakvvrrlsdspe
    retprcphfgvcggcqqqhasvdlqqrsksaalarlmkhdvseviadvpwgyrrrarlsl
    nylpktqqlqmgfrkagssdivdvkqcpilapqleallpkvraclgslqamrhlghvelv
    qatsgtlmilrhtaplssadreklerfshsegldlylapdseiletvsgempwydsnglr
    ltfsprdfiqvnagvnqkmvaralewldvqpedrvldlfcgmgnftlplatqaasvvgve
    gvpalvekgqqnarlnglqnvtfyhenleedvtkqpwakngfdkvlldparagaagvmqq
    iiklepirivyvscnpatlardseallkagytiarlamldmfphtghlesmvlfsr
    

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.