PDB entry 2bg2

View 2bg2 on RCSB PDB site
Description: Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH4.5 using 20mM ZnSO4 in the buffer. 1mM DTT and 1mM TCEP- HCl were used as reducing agents. Cys221 is reduced.
Class: hydrolase
Keywords: hydrolase, metallo-beta-lactamase, antibiotic resistance
Deposited on 2004-12-16, released 2005-03-31
The last revision prior to the SCOPe 2.07 freeze date was dated 2012-06-20, with a file datestamp of 2012-06-15.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.1787
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.07: d2bg2a_
  • Chain 'B':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.07: d2bg2b_
  • Heterogens: GOL, ZN, SO4, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2bg2A (A:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bg2A (A:)
tviknetgtisisqlnknvwvhtelgsfavpsnglvlntskglvlvdsswddkltkelie
mvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdlqt
vtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvnew
stsienvlkryrninavvpghgevgdkglllhtldllk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2bg2B (B:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bg2B (B:)
ktviknetgtisisqlnknvwvhtelgsavpsnglvlntskglvlvdsswddkltkelie
mvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdlqt
vtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvnew
stsienvlkryrninavvpghgevgdkglllhtldllk