PDB entry 2bfl

View 2bfl on RCSB PDB site
Description: bacillus cereus metallo-beta-lactamase (bcii) arg (121) cys mutant. solved at ph5 using 20mm znso4 in buffer. 1mm dtt was used as a reducing agent.
Class: hydrolase
Keywords: hydrolase, zinc, metallo-beta-lactamase, antibiotic resistance
Deposited on 2004-12-08, released 2005-03-31
The last revision prior to the SCOPe 2.01 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.1828
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.01: d2bfla_
  • Chain 'B':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.01: d2bflb_
  • Heterogens: GOL, ZN, SO4, AZI, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2bflA (A:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bflA (A:)
tviknetgtisisqlnknvwvhtelgavpsnglvlntskglvlvdsswddkltkeliemv
ekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdlqtvt
nlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvnewst
sienvlkryrninavvpghgevgdkglllhtldllk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2bflB (B:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bflB (B:)
ktviknetgtisisqlnknvwvhtelgsfgavpsnglvlntskglvlvdsswddkltkel
iemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdl
qtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvn
ewstsienvlkryrninavvpghgevgdkglllhtldllk