PDB entry 2bfc

View 2bfc on RCSB PDB site
Description: reactivity modulation of human branched-chain alpha-ketoacid dehydrogenase by an internal molecular switch
Class: oxidoreductase
Keywords: oxidoreductase, oxidative decarboxylation, maple syrup urine disease, thiamine diphosphate, phosphorylation, conformational switch
Deposited on 2004-12-06, released 2006-02-16
The last revision prior to the SCOPe 2.05 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-31.
Experiment type: XRAY
Resolution: 1.64 Å
R-factor: 0.144
AEROSPACI score: 0.61 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-oxoisovalerate dehydrogenase alpha subunit
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12694 (Start-399)
      • engineered mutation (112)
    Domains in SCOPe 2.05: d2bfca1
  • Chain 'B':
    Compound: 2-oxoisovalerate dehydrogenase beta subunit
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.05: d2bfcb1, d2bfcb2
  • Heterogens: K, MN, TZD, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2bfcA (A:)
    sslddkpqfpgasaefidklefiqpnvisgipiyrvmdrqgqiinpsedphlpkekvlkl
    yksmtllntmdrilyesqrqgrisfymtnygeegthvgsaaaldntdlvfgqfreagvlm
    yrdyplelfmaqcygnisdlgkgrqmpvhygckerhfvtissplatqipqavgaayaakr
    ananrvvicyfgegaasegdahagfnfaatlecpiiffcrnngyaistptseqyrgdgia
    argpgygimsirvdgndvfavynatkearrravaenqpflieamtyrighastsddssaf
    rsvdevnywdkqdhpisrlrhyllsqgwwdeeqekawrkqsrrkvmeafeqaerkpkpnp
    nllfsdvyqempaqlrkqqeslarhlqtygehypldhfdk
    

    Sequence, based on observed residues (ATOM records): (download)
    >2bfcA (A:)
    kpqfpgasaefidklefiqpnvisgipiyrvmdrqgqiinpsedphlpkekvlklyksmt
    llntmdrilyesqrqgrisfymtnygeegthvgsaaaldntdlvfgqfreagvlmyrdyp
    lelfmaqcygnisdlgkgrqmpvhygckerhfvtissplatqipqavgaayaakrananr
    vvicyfgegaasegdahagfnfaatlecpiiffcrnngyaistptseqyrgdgiaargpg
    ygimsirvdgndvfavynatkearrravaenqpflieamtyridhpisrlrhyllsqgww
    deeqekawrkqsrrkvmeafeqaerkpkpnpnllfsdvyqempaqlrkqqeslarhlqty
    gehypldhfdk
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >2bfcB (B:)
    vahftfqpdpepreygqtqkmnlfqsvtsaldnslakdptavifgedvafggvfrctvgl
    rdkygkdrvfntplceqgivgfgigiavtgataiaeiqfadyifpafdqivneaakyryr
    sgdlfncgsltirspwgcvghgalyhsqspeaffahcpgikvviprspfqakglllscie
    dknpciffepkilyraaaeevpiepyniplsqaeviqegsdvtlvawgtqvhvirevasm
    akeklgvscevidlrtiipwdvdticksviktgrllisheapltggfaseisstvqeecf
    lnleapisrvcgydtpfphifepfyipdkwkcydalrkminy
    

    Sequence, based on observed residues (ATOM records): (download)
    >2bfcB (B:)
    ahfeygqtqkmnlfqsvtsaldnslakdptavifgedvafggvfrctvglrdkygkdrvf
    ntplceqgivgfgigiavtgataiaeiqfadyifpafdqivneaakyryrsgdlfncgsl
    tirspwgcvghgalyhsqspeaffahcpgikvviprspfqakglllsciedknpciffep
    kilyraaaeevpiepyniplsqaeviqegsdvtlvawgtqvhvirevasmakeklgvsce
    vidlrtiipwdvdticksviktgrllisheapltggfaseisstvqeecflnleapisrv
    cgydtpfphifepfyipdkwkcydalrkminy