PDB entry 2bcj

View 2bcj on RCSB PDB site
Description: Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits
Class: transferase/hydrolase
Keywords: Peripheral membrane complex, protein kinase, RGS domain, WD40 protein, heterotrimeric G protein
Deposited on 2005-10-19, released 2005-12-20
The last revision prior to the SCOP 1.73 freeze date was dated 2005-12-20, with a file datestamp of 2007-06-28.
Experiment type: XRAY
Resolution: 3.06 Å
R-factor: 0.236
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: g-protein coupled receptor kinase 2
    Species: BOS TAURUS
    Gene: ADRBK1, GRK2
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2bcja1, d2bcja2, d2bcja3
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1
    Species: BOS TAURUS
    Gene: GNB1
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2bcjb1
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit
    Species: BOS TAURUS
    Gene: GNG2
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2bcjg1
  • Chain 'Q':
    Compound: G alpha I1, Guanine nucleotide-binding protein G(q), alpha subunit
    Species: Rattus norvegicus,Mus musculus
    Gene: Gnaq
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2bcjq1, d2bcjq2
  • Heterogens: MG, ALF, ACE, GDP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2bcjA (A:)
madleavladvsylmamekskatpaaraskkillpepsirsvmqkyledrgevtfekifs
qklgyllfrdfclkhleeakplvefyeeikkyekleteeerlvcsreifdtyimkellac
shpfsksaiehvqghlvkkqvppdlfqpyieeicqnlrgdvfqkfiesdkftrfcqwknv
elnihltmndfsvhriigrggfgevygcrkadtgkmyamkcldkkrikmkqgetlalner
imlslvstgdcpfivcmsyafhtpdklsfildlmnggdlhyhlsqhgvfseadmrfyaae
iilglehmhnrfvvyrdlkpanilldehghvrisdlglacdfskkkphasvgthgymape
vlqkgvaydssadwfslgcmlfkllrghspfrqhktkdkheidrmtltmavelpdsfspe
lrsllegllqrdvnrrlgclgrgaqevkespffrsldwqmvflqkyppplipprgevnaa
dafdigsfdeedtkgiklldsdqelyrnfpltiserwqqevaetvfdtinaetdrleark
ktknkqlgheedyalgkdcimhgymskmgnpfltqwqrryfylfpnrlewrgegeapqsl
ltmeeiqsveetqikerkclllkirggkqfvlqcdsdpelvqwkkelrdayreaqqlvqr
vpkmknkprapvvelskvpliqrgsangl

    Sequence, based on observed residues (ATOM records): (download) 
    >2bcjA (A:)
askkillpepsirsvmqkyledrgevtfekifsqklgyllfrdfclkhleeakplvefye
eikkyekleteeerlvcsreifdtyimkellacshpfsksaiehvqghlvkkqvppdlfq
pyieeicqnlrgdvfqkfiesdkftrfcqwknvelnihltmndfsvhriigrggfgevyg
crkadtgkmyamkcldkkrikmkqgetlalnerimlslvstgdcpfivcmsyafhtpdkl
sfildlmnggdlhyhlsqhgvfseadmrfyaaeiilglehmhnrfvvyrdlkpanillde
hghvrisdlglacdfskkkphasvgthgymapevlqkgvaydssadwfslgcmlfkllrg
hspfrqhktkdkheidrmtltmavelpdsfspelrsllegllqrdvnrrlgclgrgaqev
kespffrsldwqmvflqkyppplipprgdtkgiklldsdqelyrnfpltiserwqqevae
tvfdtinaetdrlearkktknkqlgheedyalgkdcimhgymskmgnpfltqwqrryfyl
fpnrlewrgegeapqslltmeeiqsveetqikerkclllkirggkqfvlqcdsdpelvqw
kkelrdayreaqqlvqrvpkmknk

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2bcjB (B:)
seldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyam
hwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnic
siynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >2bcjG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffsail

    Sequence, based on observed residues (ATOM records): (download) 
    >2bcjG (G:)
nntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfre
kkff

  • Chain 'Q':
    Sequence, based on SEQRES records: (download) 
    >2bcjQ (Q:)
mgctlsaedkaaverskmidrnlredgersrrelkllllgtgesgkstfikqmriihgsg
ysdedkrgftklvyqniftamqamiramdtlkipykyehnkahaqlvrevdvekvsafen
pyvdaikslwndpgiqecydrrreyqlsdstkyylndldrvadpsylptqqdvlrvrvpt
tgiieypfdlqsvifrmvdvggqrserrkwihcfenvtsimflvalseydqvlvesdnen
rmeeskalfrtiitypwfqnssvilflnkkdlleekimyshlvdyfpeydgpqrdaqaar
efilkmfvdlnpdsdkiiyshftcatdtenirfvfaavkdtilqlnlkeynlv

    Sequence, based on observed residues (ATOM records): (download) 
    >2bcjQ (Q:)
relkllllgtgesgkstfikqmriihgsgysdedkrgftklvyqniftamqamiramdtl
kipykyehnkahaqlvrevdvekvsafenpyvdaikslwndpgiqecydrrreyqlsdst
kyylndldrvadpsylptqqdvlrvrvpttgiieypfdlqsvifrmvdvggqrserrkwi
hcfenvtsimflvalseydqvlvesdnenrmeeskalfrtiitypwfqnssvilflnkkd
lleekimyshlvdyfpeydgpqrdaqaarefilkmfvdlnpdsdkiiyshftcatdteni
rfvfaavkdtilqlnlk