PDB entry 2b66

View 2b66 on RCSB PDB site
Description: 50S ribosomal subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400
Class: Ribosome
Keywords: ribosome, translation, release factor
Deposited on 2005-09-30, released 2006-01-10
The last revision prior to the SCOP 1.73 freeze date was dated 2006-01-10, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 5.9 Å
R-factor: 0.351
AEROSPACI score: -0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 50S ribosomal protein L27
    Species: Thermus thermophilus
  • Chain '2':
    Compound: 50S ribosomal protein L29
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b6621
  • Chain '3':
    Compound: 50S ribosomal protein L30
    Species: Thermus thermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2b6631
  • Chain '4':
    Compound: 50S ribosomal protein L31
    Species: Thermus thermophilus
  • Chain '5':
    Compound: 50S ribosomal protein L32
    Species: Thermus thermophilus
  • Chain '6':
    Compound: 50S ribosomal protein L33
    Species: Thermus thermophilus
  • Chain '7':
    Compound: 50S ribosomal protein L34
    Species: Thermus thermophilus
  • Chain '8':
    Compound: 50S ribosomal protein L35
    Species: Thermus thermophilus
  • Chain '9':
    Compound: 50S ribosomal protein L36
    Species: Thermus thermophilus
  • Chain 'A':
    Compound: 23S ribosomal RNA
    Species: Thermus thermophilus
  • Chain 'B':
    Compound: 5S ribosomal RNA
    Species: Thermus thermophilus
  • Chain 'D':
    Compound: 50S ribosomal protein L2
    Species: Thermus thermophilus
  • Chain 'E':
    Compound: 50S ribosomal protein L3
    Species: Thermus thermophilus
  • Chain 'F':
    Compound: 50S ribosomal protein L4
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66f1
  • Chain 'G':
    Compound: 50S ribosomal protein L5
    Species: Thermus thermophilus
  • Chain 'H':
    Compound: 50S ribosomal protein L6
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66h1, d2b66h2
  • Chain 'I':
    Compound: 50S ribosomal protein L9
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66i1, d2b66i2
  • Chain 'K':
    Compound: 50S ribosomal protein L11
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66k1, d2b66k2
  • Chain 'N':
    Compound: 50S ribosomal protein L13
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66n1
  • Chain 'O':
    Compound: 50S ribosomal protein L14
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66o1
  • Chain 'P':
    Compound: 50S ribosomal protein L15
    Species: Thermus thermophilus
  • Chain 'Q':
    Compound: 50S ribosomal protein L16
    Species: Thermus thermophilus
  • Chain 'R':
    Compound: 50S ribosomal protein L17
    Species: Thermus thermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2b66r1
  • Chain 'S':
    Compound: 50S ribosomal protein L18
    Species: Thermus thermophilus
  • Chain 'T':
    Compound: 50S ribosomal protein L19
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66t1
  • Chain 'U':
    Compound: 50S ribosomal protein L20
    Species: Thermus thermophilus
  • Chain 'V':
    Compound: 50S ribosomal protein L21
    Species: Thermus thermophilus
  • Chain 'W':
    Compound: 50S ribosomal protein L22
    Species: Thermus thermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2b66w1
  • Chain 'X':
    Compound: 50S ribosomal protein L23
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66x1
  • Chain 'Y':
    Compound: 50S ribosomal protein 24
    Species: Thermus thermophilus
    Domains in SCOP 1.73: d2b66y1
  • Chain 'Z':
    Compound: 50S ribosomal protein CTC
    Species: Thermus thermophilus

PDB Chain Sequences:

  • Chain '0':
    No sequence available.

  • Chain '2':
    Sequence, based on SEQRES records: (download) 
    >2b662 (2:)
tvlhvqeirdmtpaereaelddlktellnaravqaaggapenpgrikelrkaiariktiq
geegdlqene

    Sequence, based on observed residues (ATOM records): (download) 
    >2b662 (2:)
tvlhvqeirdmtpaereaelddlktellnarvqaaggapenpgrikelrkaiariktiqg
eegd

  • Chain '3':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2b663 (3:)
mprlkvklvkspigypkdqkaalkalglrrlqqervledtpairgnvekvahlvrvevve

  • Chain '4':
    No sequence available.

  • Chain '5':
    No sequence available.

  • Chain '6':
    No sequence available.

  • Chain '7':
    No sequence available.

  • Chain '8':
    No sequence available.

  • Chain '9':
    No sequence available.

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2b66F (F:)
meatiydldgntdgevdlpdvfetpvrsdligkavraaqanrkqdygsdeyaglrtpaes
fgsgrgqahvpkldgrarrvpqavkgrsahppktekdrsldlndkerqlavrsalaatad
adlvadrghefdrdevpvvvsddfedlvktqevvsllealdvhadidradetkikagqgs
argrkyrrpasilfvtsdepstaarnlagadvatasevntedlapggapgrltvftesal
aevaer

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66F (F:)
meatiydntdgevdlpdvfetpvrsdligkavraaqanrkqdygsdeyaglrtpaesfgs
grgqahrvpqavkgrsahppktekdrsldlndkerqlavrsalaatadvvvsddfedlvk
tqevvsllealdvhadidrlfvtsdepstaarnlagadvatasevntedlapgrltvfte
salaevaer

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >2b66H (H:)
srvgkkpieipagvtvtvngntvtvkgpkgeltrtfhpdmtitvegnvitvtrpsdekhh
ralhgttrsllanmvegvskgyekalelvgvgyraskqgkklvlsvgyshpveiepeegl
eievpsqtkiivkgadkqrvgelaaniravrppepykgkgiryegelvrlkegktgk

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66H (H:)
pieipagvtvtvngntvtvkgpkgeltrtfhpdmtitvegnvitvtrpsdekhhralhgt
trsllanmvegvskgyekalelvgvgyraskqgkklvlsvgyshpveiepeegleievps
qtkiivkgadkqrvgelaaniravrppepykgkgiryegelvrl

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >2b66I (I:)
mkviflkdvkgkgkkgeiknvadgyannflfkqglaieatpanlkaleaqkqkeqrqaae
elanakklkeqlekltvtipakageggrlfgsitskqiaeslqaqhglkldkrkielada
iralgytnvpvklhpevtatlkvhvteqk

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66I (I:)
mkviflkdvkgkgkkgeiknvadgyannflfkqglaieatpanlkaleaqkqkeqrqaae
elanakklkeqlekltvtipakagegrlfgsitskqiaeslqaqhglkldkrkieladai
ralgytnvpvklhpevtatlkvhvteqk

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >2b66K (K:)
makkvaaqiklqlpagkatpappvgpalgqhgvnimefckrfnaetadkagmilpvvitv
yedksftfiiktppasfllkkaagiekgssepkrkivgkvtrkqieeiaktkmpdlnans
leaamkiiegtaksmgievvd

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66K (K:)
qiklqlpagkatpappvgpalgqhgvnimefckrfnaetadkagmilpvvitvyedksft
fiiktppasfllkkaagiekgssepkrkivgkvtrkqieeiaktkmpdlnansleaamki
iegtaksmgievv

  • Chain 'N':
    Sequence, based on SEQRES records: (download) 
    >2b66N (N:)
msvaefdadvivdardcimgrvasqvaeqaldgetvavvnaeravitgreeqivekyekr
vdigndngyfypkrpdgifkrtirgmlphkkqrgreafesvrvylgnpydedgevldgts
ldrlsnikfvtlgeisetlganktw

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66N (N:)
aefdadvivdardcimgrvasqvaeqaldgetvavvnaeravitgreeqivekyekrvdi
gndngyfypkrpdgifkrtirgmlpkkqrgreafesvrvylgnpydtlgeisetlga

  • Chain 'O':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2b66O (O:)
miqqesrlkvadnsgarevlvikvlggsgrryanigdvvvatvkdatpggvvkkgqvvka
vvvrtkrgvrrpdgsyirfdenacviirddksprgtrifgpvarelrdkdfmkiislape
vi

  • Chain 'P':
    No sequence available.

  • Chain 'Q':
    No sequence available.

  • Chain 'R':
    Sequence, based on SEQRES records: (download) 
    >2b66R (R:)
mrhlksgrklnrhsshrlalyrnqaksllthgritttvpkakelrgfvdhlihlakrgdl
harrlvlrdlqdvklvrklfdeiapryrdrqggytrvlklaerrrgdgaplalvelve

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66R (R:)
sshrlalyrnqaksllthgritttvpkakelrgfvdhlihlakrgdlharrlvlrdlqdv
klvrklfdeiapryrdrqggytrvlklaerrrgdgaplalvelve

  • Chain 'S':
    No sequence available.

  • Chain 'T':
    Sequence, based on SEQRES records: (download) 
    >2b66T (T:)
prtrecdycgtdiepgtgtmfvhkdgatthfcsskcennadlgrearnlewtdtargeag
eaedea

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66T (T:)
recdycgtdiepgtgtmfvhkdgatthfcsskcennadlrearnlewtdtar

  • Chain 'U':
    No sequence available.

  • Chain 'V':
    No sequence available.

  • Chain 'W':
    Sequence, based on SEQRES records: (download) 
    >2b66W (W:)
meakaiaryvrisprkvrlvvdlirgksleearnilrytnkrgayfvakvlesaaanavn
nhdmledrlyvkaayvdegpalkrvlprargradiikkrtshitvilgekhgk

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66W (W:)
eakaiaryvrisprkvrlvvdlirgksleearnilrytnkrgayfvakvlesaaanavnn
hdledrlyvkaayvdegpalkrvlprargradiikkrtshitvilgek

  • Chain 'X':
    Sequence, based on SEQRES records: (download) 
    >2b66X (X:)
swdvikhphvtekamndmdfqnklqfavddraskgevadaveeqydvtveqvntqntmdg
ekkavvrlsedddaqevasrigvf

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66X (X:)
swdvikhphvtekamndmdfnklqfavddraskgevadaveeqydvtveqvntqntmdge
kkavvrlsedddqeva

  • Chain 'Y':
    Sequence, based on SEQRES records: (download) 
    >2b66Y (Y:)
skqpdkqrksqrraplherhkqvratlsadlreeygqrnvrvnagdtvevlrgdfageeg
evinvdldkavihvedvtlektdgeevprpldtsnvrvtdldledekrearleseddsa

    Sequence, based on observed residues (ATOM records): (download) 
    >2b66Y (Y:)
skqpdkqrksqrraplherhkqvratlsadlreeygvrvnagdtvevlrgdfageegevi
nvdldkavihvedvtlektdgeevprpldtsnvrvtdldledekrearle

  • Chain 'Z':
    No sequence available.