PDB entry 2aaf

View 2aaf on RCSB PDB site
Description: Structure of H278A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate
Class: hydrolase
Keywords: arginine degradation pathway, L-arginine deiminase, X-ray structure, catalytic mechanism
Deposited on 2005-07-13, released 2005-08-09
The last revision prior to the SCOP 1.75 freeze date was dated 2005-10-18, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.2
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Arginine deiminase
    Species: PSEUDOMONAS AERUGINOSA
    Gene: arcA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13981
      • engineered (277)
      • modified residue (405)
    Domains in SCOP 1.75: d2aafa1
  • Chain 'B':
    Compound: Arginine deiminase
    Species: PSEUDOMONAS AERUGINOSA
    Gene: arcA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13981 (Start-417)
      • engineered (277)
      • modified residue (405)
    Domains in SCOP 1.75: d2aafb1
  • Chain 'C':
    Compound: Arginine deiminase
    Species: PSEUDOMONAS AERUGINOSA
    Gene: arcA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13981 (Start-417)
      • engineered (277)
      • modified residue (405)
    Domains in SCOP 1.75: d2aafc1
  • Chain 'D':
    Compound: Arginine deiminase
    Species: PSEUDOMONAS AERUGINOSA
    Gene: arcA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13981 (Start-417)
      • engineered (277)
      • modified residue (405)
    Domains in SCOP 1.75: d2aafd1
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2aafA (A:)
mstektklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvt
kmrergidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprkla
eyliggvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpm
ywparrqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligm
gerssrqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvke
ivpfslrpdpsspygmnirreektflevvaeslglkklrvvetggnsfaaereqwddgnn
vvclepgvvvgydrntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

    Sequence, based on observed residues (ATOM records): (download) 
    >2aafA (A:)
tklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvtkmrer
gidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprklaeylig
gvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpmywpar
rqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligmgerss
rqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvkeivpfs
lrpdpsspygmnirreektflevvaeslglkklrvvetgreqwddgnnvvclepgvvvgy
drntytntllrkagvevitisaselgrgrggghcmtcpivrdpid

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2aafB (B:)
mstektklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvt
kmrergidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprkla
eyliggvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpm
ywparrqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligm
gerssrqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvke
ivpfslrpdpsspygmnirreektflevvaeslglkklrvvetggnsfaaereqwddgnn
vvclepgvvvgydrntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

    Sequence, based on observed residues (ATOM records): (download) 
    >2aafB (B:)
tklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvtkmrer
gidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprklaeylig
gvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpmywpar
rqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligmgerss
rqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvkeivpfs
lrpdpsspygmnirreektflevvaeslglkklrvvetgaaereqwddgnnvvclepgvv
vgydrntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2aafC (C:)
mstektklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvt
kmrergidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprkla
eyliggvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpm
ywparrqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligm
gerssrqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvke
ivpfslrpdpsspygmnirreektflevvaeslglkklrvvetggnsfaaereqwddgnn
vvclepgvvvgydrntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

    Sequence, based on observed residues (ATOM records): (download) 
    >2aafC (C:)
klgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvtkmrerg
idvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprklaeyligg
vaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpmywparr
qetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligmgerssr
qaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvkeivpfsl
rpdpsspygmnirreektflevvaeslglkklrvvetgereqwddgnnvvclepgvvvgy
drntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2aafD (D:)
mstektklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvt
kmrergidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprkla
eyliggvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpm
ywparrqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligm
gerssrqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvke
ivpfslrpdpsspygmnirreektflevvaeslglkklrvvetggnsfaaereqwddgnn
vvclepgvvvgydrntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy

    Sequence, based on observed residues (ATOM records): (download) 
    >2aafD (D:)
tklgvhseagklrkvmvcspglahqrltpsncdellfddviwvnqakrdhfdfvtkmrer
gidvlemhnlltetiqnpealkwildrkitadsvglgltselrswlesleprklaeylig
gvaaddlpaseganilkmyreylghssfllpplpntqftrdttcwiyggvtlnpmywpar
rqetllttaiykfhpefanaefeiwygdpdkdhgsstleggdvmpigngvvligmgerss
rqaigqvaqslfakgaaervivaglpksraamaldtvfsfcdrdlvtvfpevvkeivpfs
lrpdpsspygmnirreektflevvaeslglkklrvvetgreqwddgnnvvclepgvvvgy
drntytntllrkagvevitisaselgrgrggghcmtcpivrdpidy