PDB entry 220l

View 220l on RCSB PDB site
Description: generating ligand binding sites in t4 lysozyme using deficiency-creating substitutions
Class: hydrolase
Keywords: hydrolase, o-glycosyl, t4 lysozyme, cavity mutants, ligand binding, protein engineering, protein design
Deposited on 1997-06-25, released 1998-03-18
The last revision prior to the SCOP 1.73 freeze date was dated 2003-04-01, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: 0.149
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: t4 lysozyme
    Species: Bacteriophage T4
    Gene: GENE E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (0-End)
      • engineered (53)
      • engineered (96)
      • engineered (101)
    Domains in SCOP 1.73: d220la_
  • Heterogens: CL, BME, BNZ, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >220lA (A:)
mnifemlrideglrlkiykdtegyytigighlltkspslnaakseldkaigrntngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrraalinavfqmgetgvagftnslrm
lqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >220lA (A:)
mnifemlrideglrlkiykdtegyytigighlltkspslnaakseldkaigrntngvitk
deaeklfnqdvdaavrgilrnaklkpvydsldavrraalinavfqmgetgvagftnslrm
lqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayk