PDB entry 1y4d

View 1y4d on RCSB PDB site
Description: Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59R/E60S mutant
Class: hydrolase/hydrolase inhibitor
Keywords: serine protease; inhibitor, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Deposited on 2004-11-30, released 2005-05-17
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-10-11, with a file datestamp of 2017-10-06.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'E':
    Compound: subtilisin bpn'
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Gene: APR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-274)
      • expression tag (275)
    Domains in SCOPe 2.07: d1y4de2, d1y4de3
  • Chain 'I':
    Compound: chymotrypsin inhibitor 2
    Species: Hordeum vulgare [TaxId:4513]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q40059 (1-63)
      • engineered (39-40)
    Domains in SCOPe 2.07: d1y4di_
  • Heterogens: CA, NA, HOH

PDB Chain Sequences:

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >1y4dE (E:)
    aqsvpygvsqikapalhsqgytgsnvkvavidsgidsshpdlkvaggasmvpsetnpfqd
    nnshgthvagtvaalnnsigvlgvapsaslyavkvlgadgsgqyswiingiewaiannmd
    vinmslggpsgsaalkaavdkavasgvvvvaaagnegtsgssstvgypgkypsviavgav
    dssnqrasfssvgpeldvmapgvsiqstlpgnkygayngtsmasphvagaaalilskhpn
    wtntqvrsslentttklgdsfyygkglinvqaaaqhhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >1y4dE (E:)
    aqsvpygvsqikapalhsqgytgsnvkvavidsgidsshpdlkvaggasmvpsetnpfqd
    nnshgthvagtvaalnnsigvlgvapsaslyavkvlgadgsgqyswiingiewaiannmd
    vinmslggpsgsaalkaavdkavasgvvvvaaagnegtsgssstvgypgkypsviavgav
    dssnqrasfssvgpeldvmapgvsiqstlpgnkygayngtsmasphvagaaalilskhpn
    wtntqvrsslentttklgdsfyygkglinvqaaaqh
    

  • Chain 'I':
    Sequence, based on SEQRES records: (download)
    >1y4dI (I:)
    mktewpelvgksveeakkvilqdkpaaqiivlpvgtivtrsyridrvrlfvdrldniaqv
    prvg
    

    Sequence, based on observed residues (ATOM records): (download)
    >1y4dI (I:)
    ktewpelvgksveeakkvilqdkpaaqiivlpvgtivtrsyridrvrlfvdrldniaqvp
    rvg