PDB entry 1x86

View 1x86 on RCSB PDB site
Description: Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF in complex with RhoA
Class: signaling protein/membrane protein
Keywords: helical bundle (DH), beta sandwich (PH), alpha/beta (RhoA), signaling protein-membrane protein COMPLEX
Deposited on 2004-08-17, released 2004-09-21
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 3.22 Å
R-factor: 0.23
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Rho guanine nucleotide exchange factor 12
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGEF12, LARG, KIAA0382
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9NZN5 (Start-374)
      • engineered (209)
    Domains in SCOPe 2.08: d1x86a1, d1x86a2
  • Chain 'B':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARHA, ARH12, RHO12
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d1x86b_
  • Chain 'C':
    Compound: Rho guanine nucleotide exchange factor 12
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGEF12, LARG, KIAA0382
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9NZN5 (Start-374)
      • engineered (209)
    Domains in SCOPe 2.08: d1x86c1, d1x86c2
  • Chain 'D':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARHA, ARH12, RHO12
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d1x86d_
  • Chain 'E':
    Compound: Rho guanine nucleotide exchange factor 12
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGEF12, LARG, KIAA0382
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9NZN5 (Start-374)
      • engineered (209)
    Domains in SCOPe 2.08: d1x86e1, d1x86e2
  • Chain 'F':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARHA, ARH12, RHO12
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d1x86f_
  • Chain 'G':
    Compound: Rho guanine nucleotide exchange factor 12
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGEF12, LARG, KIAA0382
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9NZN5
      • engineered (209)
    Domains in SCOPe 2.08: d1x86g1, d1x86g2
  • Chain 'H':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARHA, ARH12, RHO12
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d1x86h_
  • Heterogens: PO4

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1x86A (A:)
gsppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregil
spselrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkha
aatfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypll
ldniakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklse
ypnveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchs
kilastadskhtfspviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvw
qdlicrmaasvkeqsvdgghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86A (A:)
ppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregilsp
selrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkhaaa
tfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypllld
niakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklseyp
nveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchstf
spviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvwqdlicrmaasvke
qs

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1x86B (B:)
gefmaairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelal
wdtagqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgn
kkdlrndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematra
alqarrgkkksgclvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86B (B:)
aairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelalwdta
gqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdl
rndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1x86C (C:)
gsppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregil
spselrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkha
aatfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypll
ldniakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklse
ypnveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchs
kilastadskhtfspviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvw
qdlicrmaasvkeqsvdgghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86C (C:)
ppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregilsp
selrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkhaaa
tfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypllld
niakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklseyp
nveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchstf
spviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvwqdlicrmaasvke
qs

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1x86D (D:)
gefmaairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelal
wdtagqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgn
kkdlrndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematra
alqarrgkkksgclvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86D (D:)
aairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelalwdta
gqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdl
rndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1x86E (E:)
gsppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregil
spselrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkha
aatfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypll
ldniakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklse
ypnveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchs
kilastadskhtfspviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvw
qdlicrmaasvkeqsvdgghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86E (E:)
ppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregilsp
selrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkhaaa
tfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypllld
niakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtveelrnldl
tkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvspviklstvlvrqvnka
lfvisqiyelvaqtvsektvwqdlicrmaasvkeqs

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >1x86F (F:)
gefmaairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelal
wdtagqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgn
kkdlrndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematra
alqarrgkkksgclvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86F (F:)
airkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelalwdtag
qedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdlr
ndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1x86G (G:)
gsppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregil
spselrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkha
aatfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypll
ldniakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrldtsslklse
ypnveelrnldltkrkmihegplvwkvnrdktidlytllledilvllqkqddrlvlrchs
kilastadskhtfspviklstvlvrqvatdnkalfvismsdngaqiyelvaqtvsektvw
qdlicrmaasvkeqsvdgghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86G (G:)
ppnwqqlvsrevllglkpceikrqevinelfyterahvrtlkvldqvfyqrvsregilsp
selrkifsnledilqlhiglneqmkavrkrnetsvidqigedlltwfsgpgeeklkhaaa
tfcsnqpfalemiksrqkkdsrfqtfvqdaesnplcrrlqlkdiiptqmqrltkypllld
niakytewpterekvkkaadhcrqilnfvnqavkeaenkqrledyqrrlltkrkmihegp
lvwdlytllledilvllqkqddrlvviklstvlvrqvatdnkalfvisqiyelvaqtvse
ktvwqdlicrmaas

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >1x86H (H:)
gefmaairkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelal
wdtagqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgn
kkdlrndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematra
alqarrgkkksgclvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1x86H (H:)
airkklvivgdgacgktcllivfskdqfpevyvptvfenyvadievdgkqvelalwdtag
qedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdlr
ndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa