PDB entry 1wsj

View 1wsj on RCSB PDB site
Description: Crystal structure of E.coli RNase HI active site mutant (K87A/H124A)
Class: hydrolase
Keywords: RNase H, active-site mutant, X-ray crystallography, hydrolase
Deposited on 2004-11-07, released 2004-11-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.235
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-154)
      • engineered (86)
      • engineered (123)
    Domains in SCOPe 2.08: d1wsja_
  • Chain 'B':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
      • engineered (123)
    Domains in SCOPe 2.08: d1wsjb_
  • Chain 'C':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
      • engineered (123)
    Domains in SCOPe 2.08: d1wsjc_
  • Chain 'D':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
    Domains in SCOPe 2.08: d1wsjd_
  • Chain 'E':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
      • engineered (123)
    Domains in SCOPe 2.08: d1wsje_
  • Chain 'F':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
    Domains in SCOPe 2.08: d1wsjf_
  • Chain 'G':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
    Domains in SCOPe 2.08: d1wsjg_
  • Chain 'H':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (86)
    Domains in SCOPe 2.08: d1wsjh_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1wsjA (A:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1wsjB (B:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjB (B:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqv

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1wsjC (C:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjC (C:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktavknvdlwqrldaalgqhqikwewvkga
aghpenercdelaraaamnptledtgyqv

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1wsjD (D:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjD (D:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vghpenercdelaraaamnptledtgyqv

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1wsjE (E:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjE (E:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqv

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >1wsjF (F:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjF (F:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkaghpenercdelaraaamnptledtgyqve

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1wsjG (G:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjG (G:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vghpenercdelaraaamnptledtgyq

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >1wsjH (H:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkgaaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wsjH (H:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmelmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkaghpenercdelaraaamnptledtgyqv