PDB entry 1wsh

View 1wsh on RCSB PDB site
Description: Crystal structure of E.coli RNase HI active site mutant (E48A/K87A)
Class: hydrolase
Keywords: RNase H, active-site mutant, X-ray crystallography
Deposited on 2004-11-07, released 2004-11-23
The last revision prior to the SCOP 1.75 freeze date was dated 2005-04-12, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: 0.223
AEROSPACI score: 0.56 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: ribonuclease hi
    Species: Escherichia coli
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4
      • engineered (47)
      • engineered (86)
    Domains in SCOP 1.75: d1wsha_
  • Chain 'B':
    Compound: ribonuclease hi
    Species: Escherichia coli
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (Start-154)
      • engineered (47)
      • engineered (86)
    Domains in SCOP 1.75: d1wshb_
  • Chain 'C':
    Compound: ribonuclease hi
    Species: Escherichia coli
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4
      • engineered (47)
      • engineered (86)
    Domains in SCOP 1.75: d1wshc_
  • Chain 'D':
    Compound: ribonuclease hi
    Species: Escherichia coli
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (Start-154)
      • engineered (47)
      • engineered (86)
    Domains in SCOP 1.75: d1wshd_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1wshA (A:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wshA (A:)
lkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealke
hcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwewv
hpenercdelaraaamnptledtgyqve

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1wshB (B:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wshB (B:)
lkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealke
hcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwewv
kgghpenercdelaraaamnptledtgyqvev

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1wshC (C:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wshC (C:)
lkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealke
hcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwewv
ghpenercdelaraaamnptledtgyq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1wshD (D:)
mlkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealk
ehcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >1wshD (D:)
lkqveiftdgsclgnpgpggygailryrgrektfsagytrttnnrmalmaaivalealke
hcevilstdsqyvrqgitqwihnwkargwktadkkpvknvdlwqrldaalgqhqikwewv
hpenercdelaraaamnptledtgyqvev