PDB entry 1w8s

View 1w8s on RCSB PDB site
Description: the mechanism of the schiff base forming fructose-1,6-bisphosphate aldolase: structural analysis of reaction intermediates
Class: lyase
Keywords: aldolase, fructose 1, 6-bisphosphate, tim barrel, glycolytic, archaeal, catalytic mechanism, reaction intermediate, lyase
Deposited on 2004-09-27, released 2005-03-23
The last revision prior to the SCOP 1.75 freeze date was dated 2005-03-23, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: 0.149
AEROSPACI score: 0.61 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sa1
  • Chain 'B':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sb1
  • Chain 'C':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sc1
  • Chain 'D':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sd1
  • Chain 'E':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8se1
  • Chain 'F':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sf1
  • Chain 'G':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sg1
  • Chain 'H':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sh1
  • Chain 'I':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8si1
  • Chain 'J':
    Compound: fructose-bisphosphate aldolase class I
    Species: Thermoproteus tenax
    Database cross-references and differences (RAF-indexed):
    • Uniprot P58315
      • engineered mutation (143)
      • engineered mutation (145)
    Domains in SCOP 1.75: d1w8sj1
  • Heterogens: FBP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1w8sA (A:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sA (A:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1w8sB (B:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sB (B:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >1w8sC (C:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sC (C:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >1w8sD (D:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sD (D:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >1w8sE (E:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sE (E:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvygg
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >1w8sF (F:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sF (F:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'G':
    Sequence, based on SEQRES records: (download)
    >1w8sG (G:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sG (G:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'H':
    Sequence, based on SEQRES records: (download)
    >1w8sH (H:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sH (H:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'I':
    Sequence, based on SEQRES records: (download)
    >1w8sI (I:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sI (I:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy
    

  • Chain 'J':
    Sequence, based on SEQRES records: (download)
    >1w8sJ (J:)
    manltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvf
    qrgiaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfe
    wkmfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkiky
    tgdpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrda
    lkfaralaelvyggkklaeplnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w8sJ (J:)
    nltekflrifarrgksiilaydhgiehgpadfmdnpdsadpeyilrlardagfdgvvfqr
    giaekyydgsvplilklngkttlyngepvsvancsveeavslgasavgytiypgsgfewk
    mfeelarikrdavkfdlplvvesfprggkvvnetapeivayaarialelgadamkikytg
    dpktfswavkvagkvpvlmsggpktkteedflkqvegvleagalgiavgrnvwqrrdalk
    faralaelvy