PDB entry 1w85

View 1w85 on RCSB PDB site
Description: the crystal structure of pyruvate dehydrogenase e1 bound to the peripheral subunit binding domain of e2
Class: oxidoreductase
Keywords: pyruvate, dehydrogenase, dihydrolipoyl, acetyl transferase, multienzyme complex, oxidoreductase, transferase
Deposited on 2004-09-16, released 2004-11-02
The last revision prior to the SCOP 1.73 freeze date was dated 2004-11-02, with a file datestamp of 2007-06-28.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.17777
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pyruvate dehydrogenase e1 component, alpha subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85a_
  • Chain 'B':
    Compound: pyruvate dehydrogenase e1 component, beta subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85b1, d1w85b2
  • Chain 'C':
    Compound: pyruvate dehydrogenase e1 component, alpha subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85c_
  • Chain 'D':
    Compound: pyruvate dehydrogenase e1 component, beta subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85d1, d1w85d2
  • Chain 'E':
    Compound: pyruvate dehydrogenase e1 component, alpha subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85e_
  • Chain 'F':
    Compound: pyruvate dehydrogenase e1 component, beta subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85f1, d1w85f2
  • Chain 'G':
    Compound: pyruvate dehydrogenase e1 component, alpha subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85g_
  • Chain 'H':
    Compound: pyruvate dehydrogenase e1 component, beta subunit
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85h1, d1w85h2
  • Chain 'I':
    Compound: dihydrolipoyllysine-residue acetyltransferase component of pyruvate
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85i_
  • Chain 'J':
    Compound: dihydrolipoyllysine-residue acetyltransferase component of pyruvate
    Species: Bacillus stearothermophilus
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d1w85j_
  • Heterogens: MG, K, TDP, PEG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1w85A (A:)
gvktfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrild
qrsislnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqafl
fsrghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgd
fyeginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdpla
vyaavkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfr
kfleakglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqye
iykekesk

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85A (A:)
tfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrildqrs
islnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqaflfsr
ghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgdfye
ginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdplavya
avkaareraingegptlietlcfrygphtmsgdskelenewakkdplvrfrkfleakglw
seeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqyeiykekesk

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1w85B (B:)
aqmtmvqaitdalrielkndpnvlifgedvgvnggvfrateglqaefgedrvfdtplaes
gigglaiglalqgfrpvpeiqffgfvyevmdsicgqmariryrtggryhmpitirspfgg
gvhtpelhsdsleglvaqqpglkvvipstpydakgllisairdndpviflehlklyrsfr
qevpegeytipigkadikregkditiiaygamvheslkaaaelekegisaevvdlrtvqp
ldietiigsvektgraivvqeaqrqagiaanvvaeinerailsleapvlrvaapdtvypf
aqaesvwlpnfkdvietakkvmnf

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1w85C (C:)
gvktfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrild
qrsislnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqafl
fsrghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgd
fyeginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdpla
vyaavkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfr
kfleakglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqye
iykekesk

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85C (C:)
tfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrildqrs
islnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqaflfsr
ghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgdfye
ginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdplavya
avkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfrkfl
eakglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqyeiyk
ekesk

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1w85D (D:)
aqmtmvqaitdalrielkndpnvlifgedvgvnggvfrateglqaefgedrvfdtplaes
gigglaiglalqgfrpvpeiqffgfvyevmdsicgqmariryrtggryhmpitirspfgg
gvhtpelhsdsleglvaqqpglkvvipstpydakgllisairdndpviflehlklyrsfr
qevpegeytipigkadikregkditiiaygamvheslkaaaelekegisaevvdlrtvqp
ldietiigsvektgraivvqeaqrqagiaanvvaeinerailsleapvlrvaapdtvypf
aqaesvwlpnfkdvietakkvmnf

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1w85E (E:)
gvktfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrild
qrsislnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqafl
fsrghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgd
fyeginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdpla
vyaavkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfr
kfleakglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqye
iykekesk

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85E (E:)
fqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrildqrsi
slnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqaflfsrg
hfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgdfyeg
infagafkapaifvvqnnrkqtvaktlaqkavaagipgiqvdgmdplavyaavkaarera
ingegptlietlcfrygphtmsgddkelenewakkdplvrfrkfleakglwseeeennvi
eqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqyeiykekesk

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1w85F (F:)
aqmtmvqaitdalrielkndpnvlifgedvgvnggvfrateglqaefgedrvfdtplaes
gigglaiglalqgfrpvpeiqffgfvyevmdsicgqmariryrtggryhmpitirspfgg
gvhtpelhsdsleglvaqqpglkvvipstpydakgllisairdndpviflehlklyrsfr
qevpegeytipigkadikregkditiiaygamvheslkaaaelekegisaevvdlrtvqp
ldietiigsvektgraivvqeaqrqagiaanvvaeinerailsleapvlrvaapdtvypf
aqaesvwlpnfkdvietakkvmnf

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1w85G (G:)
gvktfqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrild
qrsislnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqafl
fsrghfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgd
fyeginfagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdpla
vyaavkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfr
kfleakglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqye
iykekesk

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85G (G:)
fqfpfaeqlekvaeqfptfqilneegevvneeampelsdeqlkelmrrmvytrildqrsi
slnrqgrlgfyaptagqeasqiashfalekedfilpgyrdvpqiiwhglplyqaflfsrg
hfhgnqipegvnvlppqiiigaqyiqaagvalglkmrgkkavaitytgdggtsqgdfyeg
infagafkapaifvvqnnrfaistpvekqtvaktlaqkavaagipgiqvdgmdplavyaa
vkaareraingegptlietlcfrygphtmsgddptryrskelenewakkdplvrfrkfle
akglwseeeennvieqakeeikeaikkadetpkqkvtdlisimfeelpfnlkeqyeiyke
kesk

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1w85H (H:)
aqmtmvqaitdalrielkndpnvlifgedvgvnggvfrateglqaefgedrvfdtplaes
gigglaiglalqgfrpvpeiqffgfvyevmdsicgqmariryrtggryhmpitirspfgg
gvhtpelhsdsleglvaqqpglkvvipstpydakgllisairdndpviflehlklyrsfr
qevpegeytipigkadikregkditiiaygamvheslkaaaelekegisaevvdlrtvqp
ldietiigsvektgraivvqeaqrqagiaanvvaeinerailsleapvlrvaapdtvypf
aqaesvwlpnfkdvietakkvmnf

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >1w85I (I:)
agpnrrviampsvrkyarekgvdirlvqgtgkngrvlkedidaflagga

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85I (I:)
rviampsvrkyarekgvdirlvqgtgkngrvlkedidaflag

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >1w85J (J:)
agpnrrviampsvrkyarekgvdirlvqgtgkngrvlkedidaflagga

    Sequence, based on observed residues (ATOM records): (download) 
    >1w85J (J:)
viampsvrkyarekgvdigtgkngrvlkedidafl