PDB entry 1w5p

View 1w5p on RCSB PDB site
Description: stepwise introduction of zinc binding site into porphobilinogen synthase of pseudomonas aeruginosa (mutations a129c, d131c, d139c, p132e)
Class: synthase
Keywords: synthase, evolution, metalloenzyme, porphobilinogen synthase, pseudomonas aeruginosa, protein engineering,
Deposited on 2004-08-09, released 2005-01-19
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 1.55 Å
R-factor: 0.159
AEROSPACI score: 0.63 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (128)
      • engineered mutation (130-131)
      • engineered mutation (138)
      • see remark 999 (198)
    Domains in SCOPe 2.07: d1w5pa_
  • Chain 'B':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (128)
      • engineered mutation (130-131)
      • engineered mutation (138)
      • see remark 999 (198)
    Domains in SCOPe 2.07: d1w5pb_
  • Heterogens: EDO, FMT, K, MG, SO4, GOL, ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1w5pA (A:)
    msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
    erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
    elgiitdvclceftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
    rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
    ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
    laesvilesltafkragadgiltyfakqaaeqlrrgr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w5pA (A:)
    anraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsi
    dqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgii
    tdvclceftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigai
    realesaghtnvrvmaysakyasayygpfrdavnkatyqmdpansdealhevaadlaega
    dmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvilesltafk
    ragadgiltyfakqaaeqlrrg
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1w5pB (B:)
    msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
    erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
    elgiitdvclceftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
    rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
    ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
    laesvilesltafkragadgiltyfakqaaeqlrrgr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w5pB (B:)
    anraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsi
    dqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgii
    tdvclceftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigai
    realesaghtnvrvmaysakyasayygpfrdavnkatyqmdpansdealhevaadlaega
    dmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvilesltafk
    ragadgiltyfakqaaeqlrrg