PDB entry 1w5n

View 1w5n on RCSB PDB site
Description: Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C)
Class: synthase
Keywords: synthase, evolution, metalloenzyme, porphobilinogen synthase, pseudomonas aeruginosa, protein engineering
Deposited on 2004-08-09, released 2005-01-19
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-05-08, with a file datestamp of 2019-05-03.
Experiment type: XRAY
Resolution: 1.6500000000000001 Å
R-factor: N/A
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (130)
      • engineered mutation (138)
      • see remark 999 (198)
    Domains in SCOPe 2.08: d1w5na_
  • Chain 'B':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (130)
      • engineered mutation (138)
      • see remark 999 (198)
    Domains in SCOPe 2.08: d1w5nb_
  • Heterogens: FMT, CL, MG, ZN, NA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1w5nA (A:)
    msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
    erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
    elgiitdvalcpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
    rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
    ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
    laesvilesltafkragadgiltyfakqaaeqlrrgr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w5nA (A:)
    nraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsid
    qllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgiit
    dvalcpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigair
    ealesaghtnvrvmaysakyasayygpfrdavgsnkatyqmdpansdealhevaadlaeg
    admvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvilesltaf
    kragadgiltyfakqaaeqlrrg
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1w5nB (B:)
    msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
    erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
    elgiitdvalcpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
    rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
    ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
    laesvilesltafkragadgiltyfakqaaeqlrrgr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1w5nB (B:)
    anraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsi
    dqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgii
    tdvalcpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigai
    realesaghtnvrvmaysakyasayygpfrdavgsnkatyqmdpansdealhevaadlae
    gadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvileslta
    fkragadgiltyfakqaaeqlrrg