PDB entry 1w56

View 1w56 on RCSB PDB site
Description: stepwise introduction of zinc binding site into porphobilinogen synthase of pseudomonas aeruginosa (mutations a129c and d131c)
Class: synthase
Keywords: synthase, evolution, metalloenzyme, porphobilinogen synthase, pseudomonas aeruginosa, protein engineering,
Deposited on 2004-08-05, released 2005-01-19
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.14
AEROSPACI score: 0.6 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (128)
      • engineered mutation (130)
      • see remark 999 (198)
    Domains in SCOPe 2.07: d1w56a_
  • Chain 'B':
    Compound: delta-aminolevulinic acid dehydratase
    Species: PSEUDOMONAS AERUGINOSA [TaxId:208964]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (128)
      • engineered mutation (130)
      • see remark 999 (198)
    Domains in SCOPe 2.07: d1w56b_
  • Heterogens: FMT, MG, ZN, K, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1w56A (A:)
msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
elgiitdvclcpftthgqdgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
laesvilesltafkragadgiltyfakqaaeqlrrgr

    Sequence, based on observed residues (ATOM records): (download) 
    >1w56A (A:)
nraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsid
qllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgiit
dvclcpftthgqdgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigair
ealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansdealhev
aadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvi
lesltafkragadgiltyfakqaaeqlrrg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1w56B (B:)
msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
elgiitdvclcpftthgqdgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
laesvilesltafkragadgiltyfakqaaeqlrrgr

    Sequence, based on observed residues (ATOM records): (download) 
    >1w56B (B:)
anraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsi
dqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgii
tdvclcpftthgqdgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigai
realesaghtnvrvmaysakyasayygpfrdavgsnkatyqmdpansdealhevaadlae
gadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvileslta
fkragadgiltyfakqaaeqlrrg