PDB entry 1w54

View 1w54 on RCSB PDB site
Description: Stepwise introduction of a zinc binding site into Porphobilinogen synthase from Pseudomonas aeruginosa (mutation D139C)
Class: synthase
Keywords: synthase, evolution, metalloenzyme, porphobilinogen synthase, pseudomonas aeruginosa, protein engineering
Deposited on 2004-08-05, released 2005-01-19
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-05-08, with a file datestamp of 2019-05-03.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: delta-aminolevulinic acid dehydratase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (138)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d1w54a_
  • Chain 'B':
    Compound: delta-aminolevulinic acid dehydratase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q59643
      • engineered mutation (138)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d1w54b_
  • Heterogens: FMT, K, MG, ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1w54A (A:)
msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
elgiitdvaldpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
laesvilesltafkragadgiltyfakqaaeqlrrgr

    Sequence, based on observed residues (ATOM records): (download) 
    >1w54A (A:)
trlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsidqlliea
eewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgiitdvaldp
ftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigairealesa
ghtnvrvmaysakyasayygpfrdaatyqmdpansdealhevaadlaegadmvmvkpgmp
yldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvilesltafkragadgilty
fakqaaeqlrrg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1w54B (B:)
msftpanraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgv
erlsidqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfp
elgiitdvaldpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdg
rigairealesaghtnvrvmaysakyasayygpfrdavgsasnlgkgnkatyqmdpansd
ealhevaadlaegadmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngw
laesvilesltafkragadgiltyfakqaaeqlrrgr

    Sequence, based on observed residues (ATOM records): (download) 
    >1w54B (B:)
anraypytrlrrnrrddfsrrlvrenvltvddlilpvfvldgvnqresipsmpgverlsi
dqllieaeewvalgipalalfpvtpvekksldaaeaynpegiaqratralrerfpelgii
tdvaldpftthgqcgildddgyvlndvsidvlvrqalshaeagaqvvapsdmmdgrigai
realesaghtnvrvmaysakyasayygpfrdavgsatyqmdpansdealhevaadlaega
dmvmvkpgmpyldivrrvkdefraptfvyqvsgeyamhmgaiqngwlaesvilesltafk
ragadgiltyfakqaaeqlrrg