PDB entry 1um2

View 1um2 on RCSB PDB site
Description: Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment
Class: Hydrolase
Keywords: Protein splicing, Vma1-derived endonuclease, VDE, intein, extein, thiazolidine
Deposited on 2003-09-22, released 2004-09-22
The last revision prior to the SCOP 1.73 freeze date was dated 2004-09-22, with a file datestamp of 2007-07-20.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.214
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: endonuclease pi-scei
    Species: Saccharomyces cerevisiae
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (0-453)
      • engineered (0)
      • engineered (78)
    Domains in SCOP 1.73: d1um2a1, d1um2a2, d1um2a3
  • Chain 'B':
    Compound: endonuclease pi-scei
    Species: Saccharomyces cerevisiae
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (0-453)
      • engineered (0)
      • engineered (78)
    Domains in SCOP 1.73: d1um2b1, d1um2b2, d1um2b3
  • Chain 'C':
    Compound: 21-mer from Vacuolar ATP synthase catalytic subunit A
    Species: Saccharomyces cerevisiae
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (Start-10)
      • engineered (11)
    • Uniprot P17255 (11-End)
  • Chain 'D':
    Compound: 21-mer from Vacuolar ATP synthase catalytic subunit A
    Species: Saccharomyces cerevisiae
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (Start-10)
      • engineered (11)
    • Uniprot P17255 (11-End)
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1um2A (A:)
    sfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
    sdssrevpellkftcnatnelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgriv
    elvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtya
    pilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervt
    eyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgflkdgv
    knipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglv
    vsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfy
    felqelkeddyygitlsddsdhqfllanqvvvhn
    

    Sequence, based on observed residues (ATOM records): (download)
    >1um2A (A:)
    sfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqkspellkf
    tcnatnelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgrivelvkevsksypis
    egperanelvesyrkasnkayfewtieardlsllgshvrkatyqtyapilyendhffdym
    qkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervteyaeklnlcaeyk
    drkepqvaktvnlyskenplwdaivglgflkdgvknipsflstdnigtretflaglidsd
    gyvtdehgikatiktihtsvrdglvslarslglvvsvnaepisyaiymsggdvllnvlsk
    cagskkfrpapaaafarecrgfyfelqelkeddyygitlsddsdhqfllanqvvvhn
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1um2B (B:)
    sfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
    sdssrevpellkftcnatnelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgriv
    elvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtya
    pilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervt
    eyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgflkdgv
    knipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglv
    vsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfy
    felqelkeddyygitlsddsdhqfllanqvvvhn
    

    Sequence, based on observed residues (ATOM records): (download)
    >1um2B (B:)
    sfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqkellkftc
    natnelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgrivelvkevsksypiseg
    peranelvesyrkasnkayfewtieardlsllgshvrkatyqtyapilyendhffdymqk
    skfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervteyaeklnlcaeykdr
    kepqvaktvnlyslntenplwdaivglgflkdgvknipsflstdnigtretflaglidsd
    gyvtdehgikatiktihtsvrdglvslarslglvvsvnaephkisyaiymsggdvllnvl
    skcagskkfrpapaaafarecrgfyfelqelkeddyygitlsddsdhqfllanqvvvhn
    

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.