PDB entry 1tm4

View 1tm4 on RCSB PDB site
Description: crystal structure of the complex of subtilsin BPN'with chymotrypsin inhibitor 2 M59G mutant
Class: hydrolase
Keywords: serine protease, inhibitor
Deposited on 2004-06-10, released 2004-11-09
The last revision prior to the SCOP 1.75 freeze date was dated 2004-11-09, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.159
AEROSPACI score: 0.68 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'E':
    Compound: Subtilisin BPN' precursor
    Species: Bacillus amyloliquefaciens
    Gene: APR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-274)
      • his tag (275-279)
    Domains in SCOP 1.75: d1tm4e_
  • Chain 'I':
    Compound: chymotrypsin inhibitor 2
    Species: Hordeum vulgare var. distichum
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q40059 (1-63)
      • initiating methionine (0)
      • engineered (39)
    Domains in SCOP 1.75: d1tm4i_
  • Heterogens: CA, NA, CIT, 1PE, HOH

PDB Chain Sequences:

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tm4E (E:)
aqsvpygvsqikapalhsqgytgsnvkvavidsgidsshpdlkvaggasmvpsetnpfqd
nnshgthvagtvaalnnsigvlgvapsaslyavkvlgadgsgqyswiingiewaiannmd
vinmslggpsgsaalkaavdkavasgvvvvaaagnegtsgssstvgypgkypsviavgav
dssnqrasfssvgpeldvmapgvsiqstlpgnkygayngtsmasphvagaaalilskhpn
wtntqvrsslentttklgdsfyygkglinvqaaaqhhhhhh

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tm4I (I:)
mktewpelvgksveeakkvilqdkpaaqiivlpvgtivtgeyridrvrlfvdrldniaqv
prvg