PDB entry 1t4m

View 1t4m on RCSB PDB site
Description: structure of a thermostable double mutant of bacillus subtilis lipase obtained through directed evolution
Class: hydrolase
Keywords: alpha/beta hydrolase
Deposited on 2004-04-30, released 2004-11-23
The last revision prior to the SCOP 1.73 freeze date was dated 2004-11-23, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.206
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lipase a
    Species: Bacillus subtilis
    Gene: Lip A
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37957 (Start-180)
      • engineered (165)
    Domains in SCOP 1.73: d1t4ma_
  • Heterogens: K, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1t4mA (A:)
    aehnpvvmvhgiggasfnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvq
    kvldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgkalpgtdpn
    qkilytsiyssddmivmnylsrldgarnvqihgvghigllyssqvyslikeglngggqnt
    n
    

    Sequence, based on observed residues (ATOM records): (download)
    >1t4mA (A:)
    hnpvvmvhgiggasfnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvqkv
    ldetgakkvdivahsmggantlyyiknldggnkvanvvtlgganrlttgkalpgtdpnqk
    ilytsiyssddmivmnylsrldgarnvqihgvghigllyssqvyslikeglngggqntn