PDB entry 1sxj

View 1sxj on RCSB PDB site
Description: Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)
Class: replication
Keywords: clamp loader, processivity clamp, DNA sliding clamp, aaa+ ATPase, DNA polymerase, DNA-binding protein, replication
Deposited on 2004-03-30, released 2004-06-22
The last revision prior to the SCOPe 2.07 freeze date was dated 2012-02-15, with a file datestamp of 2012-02-10.
Experiment type: XRAY
Resolution: 2.85 Å
R-factor: 0.251
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Activator 1 95 kDa subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: RFC1, CDC44, YOR217W, YOR50-7
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d1sxja1, d1sxja2
  • Chain 'B':
    Compound: Activator 1 37 kDa subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: RFC4, YOL094C, O0923
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40339
      • engineered (156)
    Domains in SCOPe 2.07: d1sxjb1, d1sxjb2
  • Chain 'C':
    Compound: Activator 1 40 kDa subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: RFC3, YNL290W, N0533
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38629
      • engineered (159)
    Domains in SCOPe 2.07: d1sxjc1, d1sxjc2
  • Chain 'D':
    Compound: Activator 1 41 kDa subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: RFC2, YJR068W, J1808
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40348 (Start-352)
      • engineered (182)
    Domains in SCOPe 2.07: d1sxjd1, d1sxjd2
  • Chain 'E':
    Compound: Activator 1 40 kDa subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: RFC5, YBR087W, YBR0810
    Database cross-references and differences (RAF-indexed):
    • Uniprot P38251 (Start-353)
      • engineered (183)
    Domains in SCOPe 2.07: d1sxje1, d1sxje2
  • Chain 'F':
    Compound: Proliferating Cell Nuclear Antigen
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: POL30, YBR088C, YBR0811
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15873 (25-End)
      • expression tag (23-24)
      • modified residue (25)
      • modified residue (94)
      • modified residue (143)
      • modified residue (185)
      • modified residue (212)
      • modified residue (223)
    Domains in SCOPe 2.07: d1sxjf1, d1sxjf2, d1sxjf3
  • Chain 'G':
    Compound: Proliferating Cell Nuclear Antigen
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: POL30, YBR088C, YBR0811
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15873 (25-End)
      • expression tag (22-24)
      • modified residue (25)
      • modified residue (94)
      • modified residue (143)
      • modified residue (185)
      • modified residue (212)
      • modified residue (223)
    Domains in SCOPe 2.07: d1sxjg1, d1sxjg2, d1sxjg3
  • Chain 'H':
    Compound: Proliferating Cell Nuclear Antigen
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: POL30, YBR088C, YBR0811
    Database cross-references and differences (RAF-indexed):
    • Uniprot P15873 (25-End)
      • expression tag (12-24)
      • modified residue (25)
      • modified residue (94)
      • modified residue (143)
      • modified residue (185)
      • modified residue (212)
      • modified residue (223)
    Domains in SCOPe 2.07: d1sxjh1, d1sxjh2, d1sxjh3
  • Heterogens: MG, AGS, ADP

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1sxjA (A:)
mgsshhhhhhssglevlfqgphmasdklwtvkyaptnlqqvcgnkgsvmklknwlanwen
skknsfkhagkdgsgvfraamlygppgigkttaahlvaqelgydileqnasdvrsktlln
agvknaldnmsvvgyfkhneeaqnlngkhfviimdevdgmsggdrggvgqlaqfcrktst
plilicnernlpkmrpfdrvcldiqfrrpdansiksrlmtiairekfkldpnvidrliqt
trgdirqvinllstistttktinhenineiskaweknialkpfdiahkmldgqiysdigs
rnftlndkialyfddfdftplmiqenylstrpsvlkpgqshleavaeaancislgdivek
kirsseqlwsllplhavlssvypaskvaghmagrinftawlgqnsksakyyrllqeihyh
trlgtstxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxff
vgpdvttaiikkipatvksgftrkynsmthpvaiyr

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjA (A:)
dklwtvkyaptnlqqvcgnkgsvmklknwlanwenskknsfkhagkdgsgvfraamlygp
pgigkttaahlvaqelgydileqnasdvrsktllnagvknaldnmsvvgyfkhqnlngkh
fviimdevdgmsggdrggvgqlaqfcrktstplilicnernlpkmrpfdrvcldiqfrrp
dansiksrlmtiairekfkldpnvidrliqttrgdirqvinllstistttktinhenine
iskaweknialkpfdiahkmldgqiysdigsrnftlndkialyfddfdftplmiqenyls
trpsvlkpgqshleavaeaancislgdivekkirsseqlwsllplhavlssvypaskvag
hmagrinftawlgqnsksakyyrllqeihyhtrlgxxxxxxxxxxxxxxxxxxxxxxxxx
xxxxxxxxxxxxxxxxxxxxx

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1sxjB (B:)
msktlslqlpwvekyrpqvlsdivgnketidrlqqiakdgnmphmiisgmpgigkttsvh
clahellgrsyadgvlelnasddrgidvvrnqikhfaqkklhlppgkhkivildeadsmt
agaqqalrrtmelysnstrfafacnqsnkiieplqsqcailrysklsdedvlkrllqiik
ledvkytndgleaiiftaegdmrqainnlqstvaghglvnadnvfkivdsphplivkkml
lasnledsiqilrtdlwkkgyssidivttsfrvtknlaqvkesvrlemikeiglthmril
egvgtylqlasmlakihklnnka

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjB (B:)
lqlpwvekyrpqvlsdivgnketidrlqqiakdgnmphmiisgmpgigkttsvhclahel
lgrsyadgvlelnasddrgidvvrnqikhfaqkklhlppgkhkivildeadsmtagaqqa
lrrtmelysnstrfafacnqsnkiieplqsqcailrysklsdedvlkrllqiikledvky
tndgleaiiftaegdmrqainnlqstvaghglvnadnvfkivdsphplivkkmllasnle
dsiqilrtdlwkkgyssidivttsfrvtknlaqvkesvrlemikeiglthmrilegvgty
lqlasmlakihklnnk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1sxjC (C:)
mststekrskenlpwvekyrpetldevygqnevittvrkfvdegklphllfygppgtgkt
stivalareiygknysnmvlelnasddrgidvvrnqikdfastrqifskgfkliildead
amtnaaqnalrrvierytkntrfcvlanyahkltpallsqctrfrfqplpqeaierrian
vlvheklklspnaekalielsngdmrrvlnvlqsckatldnpdedeisddviyeccgapr
psdlkavlksileddwgtahytlnkvrsakglalidliegivkiledyelqneetrvhll
tkladieysiskggndqiqgsavigaikasfenetvkanv

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjC (C:)
nlpwvekyrpetldevygqnevittvrkfvdegklphllfygppgtgktstivalareiy
gknysnmvlelnasddrgidvvrnqikdfastrqifskgfkliildeadamtnaaqnalr
rvierytkntrfcvlanyahkltpallsqctrfrfqplpqeaierrianvlvheklklsp
naekalielsngdmrrvlnvlqsckatldnpdedeisddviyeccgaprpsdlkavlksi
leddwgtahytlnkvrsakglalidliegivkiledyelqneetrvhlltkladieysis
kggndqiqgsavigaikasfen

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1sxjD (D:)
mfegfgpnkkrkisklaaeqslaqqpwvekyrpknldevtaqdhavtvlkktlksanlph
mlfygppgtgktstilaltkelygpdlmksrilelnasdergisivrekvknfarltvsk
pskhdlenypcppykiiildeadsmtadaqsalrrtmetysgvtrfclicnyvtriidpl
asqcskfrfkaldasnaidrlrfiseqenvkcddgvlerildisagdlrrgitllqsask
gaqylgdgknitstqveelagvvphdilieivekvksgdfdeikkyvntfmksgwsaasv
vnqlheyyitndnfdtnfknqiswllfttdsrlnngtnehiqllnllvkisql

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjD (D:)
pwvekyrpknldevtaqdhavtvlkktlksanlphmlfygppgtgktstilaltkelygp
dlmksrilelnasdergisivrekvknfarltvskpskhdlenypcppykiiildeadsm
tadaqsalrrtmetysgvtrfclicnyvtriidplasqcskfrfkaldasnaidrlrfis
eqenvkcddgvlerildisagdlrrgitllqsaskgaqylgdgknitstqveelagvvph
dilieivekvksgdfdeikkyvntfmksgwsaasvvnqlheyyitndnfdtnfknqiswl
lfttdsrlnngtnehiqllnllvkisql

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1sxjE (E:)
mslwvdkyrpkslnalshneeltnflkslsdqprdlphlllygpngtgkktrcmallesi
fgpgvyrlkidvrqfvtasnrklelnvvsspyhleitpsdmgnndriviqellkevaqme
qvdfqdskdglahrykcviineansltkdaqaalrrtmekysknirlimvcdsmspiiap
iksqcllircpapsdseistilsdvvtneriqletkdilkriaqasngnlrvsllmlesm
alnnelalkssspiikpdwiivihkltrkivkersvnsliecravlydllahcipaniil
keltfslldvetlnttnkssiieyssvfderlslgnkaifhlegfiakvmccld

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjE (E:)
wvdkyrpkslnalshneeltnflkslsdqprdlphlllygpngtgkktrcmallesifgp
gvyrnvvsspyhleitpsndriviqellkevaqmeqrykcviineansltkdaqaalrrt
mekysknirlimvcdsmspiiapiksqcllircpapsdseistilsdvvtneriqletkd
ilkriaqasngnlrvsllmlesmalnnelalkssspiikpdwiivihkltrkivkersvn
sliecravlydllahcipaniilkeltfslldvetlnttnkssiieyssvfderlslgnk
aifhlegfiakvmccld

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >1sxjF (F:)
mgsshhhhhhssglevlfqgphmasmleakfeeaslfkriidgfkdcvqlvnfqckedgi
iaqavddsrvllvsleigveafqeyrcdhpvtlgmdltslskilrcgnntdtltliadnt
pdsiillfedtkkdriaeyslklmdidadflkieelqydstlslpssefskivrdlsqls
dsinimitketikfvadgdigsgsviikpfvdmehpetsiklemdqpvdltfgakylldi
ikgsslsdrvgirlsseapalfqfdlksgflqfflapkfndee

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjF (F:)
asmleakfeeaslfkriidgfkdcvqlvnfqckedgiiaqavddsrvllvsleigveafq
eyrcdhpvtlgmdltslskilrcgnntdtltliadntpdsiillfedtkkdriaeyslkl
mdidadflkieelqydstlslpssefskivrdlsqlsdsinimitketikfvadgdigsg
sviikpfvdmehpetsiklemdqpvdltfgakylldiikgsslsdrvgirlsseapalfq
fdlksgflqfflapkfnd

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1sxjG (G:)
mgsshhhhhhssglevlfqgphmasmleakfeeaslfkriidgfkdcvqlvnfqckedgi
iaqavddsrvllvsleigveafqeyrcdhpvtlgmdltslskilrcgnntdtltliadnt
pdsiillfedtkkdriaeyslklmdidadflkieelqydstlslpssefskivrdlsqls
dsinimitketikfvadgdigsgsviikpfvdmehpetsiklemdqpvdltfgakylldi
ikgsslsdrvgirlsseapalfqfdlksgflqfflapkfndee

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjG (G:)
masmleakfeeaslfkriidgfkdcvqlvnfqckedgiiaqavddsrvllvsleigveaf
qeyrcdhpvtlgmdltslskilrcgnntdtltliadntpdsiillfedtkkdriaeyslk
lmdidadflkieelqydstlslpssefskivrdlsqlsdsinimitketikfvadgdigs
gsviikpfvdmehpetsiklemdqpvdltfgakylldiikgsslsdrvgirlsseapalf
qfdlksgflqfflapkfn

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >1sxjH (H:)
mgsshhhhhhssglevlfqgphmasmleakfeeaslfkriidgfkdcvqlvnfqckedgi
iaqavddsrvllvsleigveafqeyrcdhpvtlgmdltslskilrcgnntdtltliadnt
pdsiillfedtkkdriaeyslklmdidadflkieelqydstlslpssefskivrdlsqls
dsinimitketikfvadgdigsgsviikpfvdmehpetsiklemdqpvdltfgakylldi
ikgsslsdrvgirlsseapalfqfdlksgflqfflapkfndee

    Sequence, based on observed residues (ATOM records): (download) 
    >1sxjH (H:)
glevlfqgphmasmleakfeeaslfkriidgfkdcvqlvnfqckedgiiaqavddsrvll
vsleigveafqeyrcdhpvtlgmdltslskilrcgnntdtltliadntpdsiillfedtk
kdriaeyslklmdidadflkieelqydstlslpssefskivrdlsqlsdsinimitketi
kfvadgdigsgsviikpfvdmehpetsiklemdqpvdltfgakylldiikgsslsdrvgi
rlsseapalfqfdlksgflqfflapkf