PDB entry 1stx

View 1stx on RCSB PDB site
Description: Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+
Class: hydrolase/DNA
Keywords: restriction enzyme; EcoRV; DNA; X-ray crystallography, HYDROLASE-DNA COMPLEX
Deposited on 2004-03-25, released 2004-04-06
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-11, with a file datestamp of 2017-10-06.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Type II restriction enzyme EcoRV
    Species: Escherichia coli [TaxId:562]
    Gene: ecoRVR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04390 (0-243)
      • engineered (36)
    Domains in SCOPe 2.08: d1stxa_
  • Chain 'B':
    Compound: Type II restriction enzyme EcoRV
    Species: Escherichia coli [TaxId:562]
    Gene: ecoRVR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04390 (0-243)
      • engineered (36)
    Domains in SCOPe 2.08: d1stxb_
  • Chain 'C':
    Compound: 5'-d(*ap*ap*ap*gp*ap*t)-3'
  • Chain 'D':
    Compound: 5'-d(p*ap*tp*cp*tp*t)-3'
  • Chain 'E':
    Compound: 5'-d(*ap*ap*ap*gp*ap*t)-3'
  • Chain 'F':
    Compound: 5'-d(p*ap*tp*cp*tp*t)-3'
  • Heterogens: MN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1stxA (A:)
slrsdlinalydenqkydvcgiisaegkiyplgsdtavlstifelfsrpiinkiaekhgy
iveepkqqnhypdftlykpsepnkkiaidikttytnkenekikftlggytsfirnntkni
vypfdqyiahwiigyvytrvatrksslktyninelneipkpykgvkvflqdkwviagdla
gsgnttnigsihahykdfvegkgifdsedefldywrnyertsqlrndkynniseyrnwiy
rgrk

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1stxB (B:)
slrsdlinalydenqkydvcgiisaegkiyplgsdtavlstifelfsrpiinkiaekhgy
iveepkqqnhypdftlykpsepnkkiaidikttytnkenekikftlggytsfirnntkni
vypfdqyiahwiigyvytrvatrksslktyninelneipkpykgvkvflqdkwviagdla
gsgnttnigsihahykdfvegkgifdsedefldywrnyertsqlrndkynniseyrnwiy
rgrk

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.