PDB entry 1sku

View 1sku on RCSB PDB site
Description: E. coli Aspartate Transcarbamylase 240's Loop Mutant (K244N)
Class: transferase
Keywords: allosteric enzyme, loop movements, small-angle X-ray scattering, domain closure, allosteric transition, intersubunit interactions, TRANSFERASE
Deposited on 2004-03-05, released 2004-03-30
The last revision prior to the SCOPe 2.06 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: 0.202
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aspartate carbamoyltransferase catalytic chain
    Species: Escherichia coli [TaxId:562]
    Gene: PYRB, B4245, C5345, Z5856, ECS5222, SF4245, S4507
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A786 (0-309)
      • engineered (243)
    Domains in SCOPe 2.06: d1skua1, d1skua2
  • Chain 'B':
    Compound: Aspartate carbamoyltransferase regulatory chain
    Species: Escherichia coli [TaxId:562]
    Gene: PYRI, B4244, C5344, Z5855, ECS5221
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d1skub1, d1skub2
  • Chain 'C':
    Compound: Aspartate carbamoyltransferase catalytic chain
    Species: Escherichia coli [TaxId:562]
    Gene: PYRB, B4245, C5345, Z5856, ECS5222, SF4245, S4507
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A786 (0-309)
      • engineered (243)
    Domains in SCOPe 2.06: d1skuc1, d1skuc2
  • Chain 'D':
    Compound: Aspartate carbamoyltransferase regulatory chain
    Species: Escherichia coli [TaxId:562]
    Gene: PYRI, B4244, C5344, Z5855, ECS5221
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d1skud1, d1skud2
  • Heterogens: ZN, MLI, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1skuA (A:)
anplyqkhiisindlsrddlnlvlataaklkanpqpellkhkviascffeastrtrlsfe
tsmhrlgasvvgfsdsantslgkkgetladtisvistyvdaivmrhpqegaarlatefsg
nvpvlnagdgsnqhptqtlldlftiqetqgrldnlhvamvgdlkygrtvhsltqalakfd
gnrfyfiapdalampqyildmldekgiawslhssieevmaevdilymtrvqkerldpsey
anvnaqfvlrasdlhnakanmkvlhplprvdeiatdvdktphawyfqqagngifarqall
alvlnrdlvl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1skuB (B:)
mthdnklqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlik
ientflsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaep
vsssfavrkrandialkckycekefshnvvlan

    Sequence, based on observed residues (ATOM records): (download) 
    >1skuB (B:)
klqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlikientf
lsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaepvsssf
avrkrandialkckycekefshnvvlan

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1skuC (C:)
anplyqkhiisindlsrddlnlvlataaklkanpqpellkhkviascffeastrtrlsfe
tsmhrlgasvvgfsdsantslgkkgetladtisvistyvdaivmrhpqegaarlatefsg
nvpvlnagdgsnqhptqtlldlftiqetqgrldnlhvamvgdlkygrtvhsltqalakfd
gnrfyfiapdalampqyildmldekgiawslhssieevmaevdilymtrvqkerldpsey
anvnaqfvlrasdlhnakanmkvlhplprvdeiatdvdktphawyfqqagngifarqall
alvlnrdlvl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1skuD (D:)
mthdnklqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlik
ientflsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaep
vsssfavrkrandialkckycekefshnvvlan

    Sequence, based on observed residues (ATOM records): (download) 
    >1skuD (D:)
klqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlikientf
lsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaepvsssf
avrkrandialkckycekefshnvvlan