PDB entry 1s46

View 1s46 on RCSB PDB site
Description: Covalent intermediate of the E328Q amylosucrase mutant
Class: transferase
Keywords: protein-glucopyranosyl covalent intermediate, (beta/alpha)8-barrel, TRANSFERASE
Deposited on 2004-01-15, released 2004-03-23
The last revision prior to the SCOPe 2.05 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.184
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: amylosucrase
    Species: Neisseria polysaccharea [TaxId:489]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9ZEU2 (4-627)
      • cloning artifact (0-3)
      • see remark 999 (130)
      • engineered (327)
    Domains in SCOPe 2.05: d1s46a1, d1s46a2
  • Heterogens: BGC, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1s46A (A:)
spnsqylktrildiytpeqragieksedwrqfsrrmdthfpklmneldsvygnneallpm
lemllaqawqsysqrnsslkdidiarennpdwilsnkqvggvcyvdlfagdlkglkdkip
yfqelgltylylmplfkcpegksdggyavssyrdvnpalgtigdlreviaalheagisav
vdfifnhtsnehewaqrcaagdplfdnfyyifpdrrmpdqydrtlreifpdqhpggfsql
edgrwvwttfnsfqwdlnysnpwvframagemlflanlgvdilrmdavafiwkqmgtsce
nlpqahalirafnavmriaapavffksqaivhpdqvvqyigqdecqigynplqmallwnt
latrevnllhqaltyrhnlpehtawvnyvrshddigwtfadedaaylgisgydhrqflnr
ffvnrfdgsfargvpfqynpstgdcrvsgtaaalvglaqddphavdrikllysialstgg
lpliylgdevgtlndddwsqdsnksddsrwahrprynealyaqrndpstaagqiyqglrh
miavrqsnprfdggrlvtfntnnkhiigyirnnallafgnfseypqtvtahtlqampfka
hdliggktvslnqdltlqpyqvmwleia