PDB entry 1rwb

View 1rwb on RCSB PDB site
Description: Cooperative Effect of Two Surface Amino Acid Mutations (Q252L and E170K) of Glucose Dehydrogenase from Bacillus megaterium IWG3 for the stabilization of Oligomeric State
Class: oxidoreductase
Keywords: oxidoreductase
Deposited on 2003-12-16, released 2003-12-30
The last revision prior to the SCOP 1.75 freeze date was dated 2005-06-21, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.224
AEROSPACI score: 0.49 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glucose 1-dehydrogenase
    Species: Bacillus megaterium
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40288 (0-260)
      • engineered (169)
      • engineered (251)
    Domains in SCOP 1.75: d1rwba_
  • Chain 'B':
    Compound: glucose 1-dehydrogenase
    Species: Bacillus megaterium
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40288 (0-260)
      • engineered (169)
      • engineered (251)
    Domains in SCOP 1.75: d1rwbb_
  • Chain 'E':
    Compound: glucose 1-dehydrogenase
    Species: Bacillus megaterium
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40288 (0-260)
      • engineered (169)
      • engineered (251)
    Domains in SCOP 1.75: d1rwbe_
  • Chain 'F':
    Compound: glucose 1-dehydrogenase
    Species: Bacillus megaterium
    Database cross-references and differences (RAF-indexed):
    • Uniprot P40288 (0-260)
      • engineered (169)
      • engineered (251)
    Domains in SCOP 1.75: d1rwbf_
  • Heterogens: NAD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1rwbA (A:)
mykdlegkvvvitgsstglgksmairfatekakvvvnyrskedeansvleeikkvggeai
avkgdvtvesdvinlvqsaikefgkldvminnaglenpvsshemslsdwnkvidtnltga
flgsreaikyfvendikgtvinmssvhekipwplfvhyaaskggmklmtktlaleyapkg
irvnnigpgaintpinaekfadpeqradvesmipmgyigepeeiaavaawlasseasyvt
gitlfadggmtlypsfqagrg

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1rwbB (B:)
mykdlegkvvvitgsstglgksmairfatekakvvvnyrskedeansvleeikkvggeai
avkgdvtvesdvinlvqsaikefgkldvminnaglenpvsshemslsdwnkvidtnltga
flgsreaikyfvendikgtvinmssvhekipwplfvhyaaskggmklmtktlaleyapkg
irvnnigpgaintpinaekfadpeqradvesmipmgyigepeeiaavaawlasseasyvt
gitlfadggmtlypsfqagrg

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1rwbE (E:)
mykdlegkvvvitgsstglgksmairfatekakvvvnyrskedeansvleeikkvggeai
avkgdvtvesdvinlvqsaikefgkldvminnaglenpvsshemslsdwnkvidtnltga
flgsreaikyfvendikgtvinmssvhekipwplfvhyaaskggmklmtktlaleyapkg
irvnnigpgaintpinaekfadpeqradvesmipmgyigepeeiaavaawlasseasyvt
gitlfadggmtlypsfqagrg

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1rwbF (F:)
mykdlegkvvvitgsstglgksmairfatekakvvvnyrskedeansvleeikkvggeai
avkgdvtvesdvinlvqsaikefgkldvminnaglenpvsshemslsdwnkvidtnltga
flgsreaikyfvendikgtvinmssvhekipwplfvhyaaskggmklmtktlaleyapkg
irvnnigpgaintpinaekfadpeqradvesmipmgyigepeeiaavaawlasseasyvt
gitlfadggmtlypsfqagrg