PDB entry 1pvp

View 1pvp on RCSB PDB site
Description: basis for a switch in substrate specificity: crystal structure of selected variant of cre site-specific recombinase, alshg bound to the engineered recognition site loxm7
Class: recombination/DNA
Keywords: Cre, recombinase, DNA, CRE-LOXP RECOMBINATION, RECOMBINATION-DNA COMPLEX
Deposited on 2003-06-28, released 2004-02-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-04-07, with a file datestamp of 2021-04-02.
Experiment type: XRAY
Resolution: 2.35 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Recombinase cre
    Species: Escherichia phage P1 [TaxId:10678]
    Gene: cre
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06956
      • engineered mutation (179)
      • engineered mutation (263-264)
      • engineered mutation (267)
      • engineered mutation (271)
    Domains in SCOPe 2.08: d1pvpa1, d1pvpa2
  • Chain 'B':
    Compound: Recombinase cre
    Species: Escherichia phage P1 [TaxId:10678]
    Gene: cre
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06956
      • engineered mutation (179)
      • engineered mutation (263-264)
      • engineered mutation (267)
      • engineered mutation (271)
    Domains in SCOPe 2.08: d1pvpb1, d1pvpb2
  • Chain 'C':
    Compound: 34-mer
    Species: Escherichia virus P1, synthetic [TaxId:10678]
  • Chain 'D':
    Compound: 34-mer
    Species: Escherichia virus P1, synthetic [TaxId:10678]
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1pvpA (A:)
mhhhhhhsnlltvhqnlpalpvdatsdevrknlmdmfrdrqafsehtwkmllsvcrswaa
wcklnnrkwfpaepedvrdyllylqarglavktiqqhlgqlnmlhrrsglprpsdsnavs
lvmrrirkenvdagerakqalafertdfdqvrslmensdrcqdirnlaflgiayntllra
aeiarirvkdisrtdggrmlihigrtktlvstagvekalslgvtklverwisvsgvaddp
nnylfcrvrkngvaapsatsqlslsalhgifgathrliygakddsgqrylawsghsarvg
aardmaragvsipeimqaggwtnvnivmnyirnldsetgamvrlledgd

    Sequence, based on observed residues (ATOM records): (download) 
    >1pvpA (A:)
tsdevrknlmdmfrdrqafsehtwkmllsvcrswaawcklnnrkwfpaepedvrdyllyl
qarglavktiqqhlgqlnmlhrrsglprpsdsnavslvmrrirkenvdagerakqalafe
rtdfdqvrslmensdrcqdirnlaflgiayntllraaeiarirvkdisrtdggrmlihig
rtktlvstagvekalslgvtklverwisvsgvaddpnnylfcrvrkngvaapsatsqlsl
salhgifgathrliygakddsgqrylawsghsarvgaardmaragvsipeimqaggwtnv
nivmnyirnldsetgamvrlled

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1pvpB (B:)
mhhhhhhsnlltvhqnlpalpvdatsdevrknlmdmfrdrqafsehtwkmllsvcrswaa
wcklnnrkwfpaepedvrdyllylqarglavktiqqhlgqlnmlhrrsglprpsdsnavs
lvmrrirkenvdagerakqalafertdfdqvrslmensdrcqdirnlaflgiayntllra
aeiarirvkdisrtdggrmlihigrtktlvstagvekalslgvtklverwisvsgvaddp
nnylfcrvrkngvaapsatsqlslsalhgifgathrliygakddsgqrylawsghsarvg
aardmaragvsipeimqaggwtnvnivmnyirnldsetgamvrlledgd

    Sequence, based on observed residues (ATOM records): (download) 
    >1pvpB (B:)
tsdevrknlmdmfrdrqafsehtwkmllsvcrswaawcklnnrkwfpaepedvrdyllyl
qarglavktiqqhlgqlnmlhrrsglprpsdsnavslvmrrirkenvdagerakqalafe
rtdfdqvrslmensdrcqdirnlaflgiayntllraaeiarirvkdisrtdggrmlihig
rtktlvstagvekalslgvtklverwisvsgvaddpnnylfcrvrkngvaapsatsqlsl
salhgifgathrliygakddsgqrylawsghsarvgaardmaragvsipeimqaggwtnv
nivmnyirtgamvrlled

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.