PDB entry 1ni0
View 1ni0 on RCSB PDB site
Description: Structure of the Y94F mutant of the restriction endonuclease PvuII
Class: hydrolase
Keywords: Restriction endonuclease, PvuII, Point mutation, HYDROLASE
Deposited on
2002-12-20, released
2003-02-04
The last revision prior to the SCOPe 2.07 freeze date was dated
2009-02-24, with a file datestamp of
2009-02-03.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.228
AEROSPACI score: 0.28
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: type II restriction enzyme pvuii
Species: Proteus vulgaris [TaxId:585]
Gene: PvuiiR
Database cross-references and differences (RAF-indexed):
- Uniprot P23657 (Start-156)
- engineered (93)
- expression tag (157-158)
Domains in SCOPe 2.07: d1ni0a1, d1ni0a2 - Chain 'B':
Compound: type II restriction enzyme pvuii
Species: Proteus vulgaris [TaxId:585]
Gene: PvuiiR
Database cross-references and differences (RAF-indexed):
- Uniprot P23657 (Start-156)
- engineered (93)
- expression tag (157-158)
Domains in SCOPe 2.07: d1ni0b1, d1ni0b2 - Chain 'C':
Compound: type II restriction enzyme pvuii
Species: Proteus vulgaris [TaxId:585]
Gene: PvuiiR
Database cross-references and differences (RAF-indexed):
- Uniprot P23657 (Start-156)
- engineered (93)
- expression tag (157-158)
Domains in SCOPe 2.07: d1ni0c1, d1ni0c2 - Heterogens: HOH
PDB Chain Sequences:
- Chain 'A':
Sequence, based on SEQRES records: (download)
>1ni0A (A:)
mshpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndav
dnagqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdl
efyydkwerkwysdghkdinnpkipvkyvmehgtkiyaa
Sequence, based on observed residues (ATOM records): (download)
>1ni0A (A:)
hpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndavdn
agqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdlef
yydkwerkwysdghkdinnpkipvkyvmehgtkiyaa
- Chain 'B':
Sequence, based on SEQRES records: (download)
>1ni0B (B:)
mshpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndav
dnagqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdl
efyydkwerkwysdghkdinnpkipvkyvmehgtkiyaa
Sequence, based on observed residues (ATOM records): (download)
>1ni0B (B:)
hpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndavdn
agqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdlef
yydkwerkwysdghkdinnpkipvkyvmehgtkiyaa
- Chain 'C':
Sequence, based on SEQRES records: (download)
>1ni0C (C:)
mshpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndav
dnagqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdl
efyydkwerkwysdghkdinnpkipvkyvmehgtkiyaa
Sequence, based on observed residues (ATOM records): (download)
>1ni0C (C:)
shpdlnkllelwphiqeyqdlalkhgindifqdnggkllqvllitgltvlpgregndavd
nagqeyelksinidltkgfsthhhmnpviiakfrqvpwifaiyrgiaieaiyrlepkdle
fyydkwerkwysdghkdinnpkipvkyvmehgtkiyaa