PDB entry 1n3e

View 1n3e on RCSB PDB site
Description: Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)
Class: hydrolase/DNA
Keywords: Homing, endonuclease, LAGLIDADG, DNA recognition, HYDROLASE/DNA COMPLEX
Deposited on 2002-10-28, released 2003-06-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.219
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Gene: CR.LSU INTRON OF CHOROPLAST 23S RDNA GENE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725
      • see remark 999 (41)
      • see remark 999 (109-110)
    Domains in SCOPe 2.08: d1n3ea_
  • Chain 'B':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Gene: CR.LSU INTRON OF CHOROPLAST 23S RDNA GENE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725
      • see remark 999 (41)
      • see remark 999 (109-110)
    Domains in SCOPe 2.08: d1n3eb_
  • Chain 'C':
    Compound: 5'-d(*cp*gp*ap*ap*ap*ap*cp*gp*tp*cp*gp*tp*ap*c)-3'
  • Chain 'D':
    Compound: 5'-d(p*gp*ap*cp*gp*tp*tp*tp*tp*cp*g)-3'
  • Chain 'E':
    Compound: 5'-d(*cp*gp*ap*ap*ap*ap*cp*gp*tp*cp*gp*tp*ap*c)-3'
  • Chain 'F':
    Compound: 5'-d(p*gp*ap*cp*gp*tp*tp*tp*tp*cp*g)-3'
  • Chain 'G':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Gene: CR.LSU INTRON OF CHOROPLAST 23S RDNA GENE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725
      • see remark 999 (41)
      • see remark 999 (109-110)
    Domains in SCOPe 2.08: d1n3eg_
  • Chain 'H':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Gene: CR.LSU INTRON OF CHOROPLAST 23S RDNA GENE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725
      • see remark 999 (41)
      • see remark 999 (109-110)
    Domains in SCOPe 2.08: d1n3eh_
  • Chain 'I':
    Compound: 5'-d(*cp*gp*ap*ap*ap*ap*cp*gp*tp*cp*gp*tp*ap*c)-3'
  • Chain 'J':
    Compound: 5'-d(p*gp*ap*cp*gp*tp*tp*tp*tp*cp*g)-3'
  • Chain 'K':
    Compound: 5'-d(*cp*gp*ap*ap*ap*ap*cp*gp*tp*cp*gp*tp*ap*c)-3'
  • Chain 'L':
    Compound: 5'-d(p*gp*ap*cp*gp*tp*tp*tp*tp*cp*g)-3'
  • Heterogens: CA, NA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1n3eA (A:)
mntkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1n3eA (A:)
tkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvdei
gvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkf
levctwvdqiaalndsktrkttsetvravld

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1n3eB (B:)
mntkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1n3eB (B:)
tkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvdei
gvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkf
levctwvdqiaalndsktrkttsetvravld

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1n3eG (G:)
mntkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1n3eG (G:)
tkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvdei
gvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkf
levctwvdqiaalndsktrkttsetvravld

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >1n3eH (H:)
mntkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1n3eH (H:)
tkynkefllylagfvdgdgsiiaqikpnqsykfkhqlsltfqvtqktqrrwfldklvdei
gvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkf
levctwvdqiaalndsktrkttsetvravld

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.