PDB entry 1mow

View 1mow on RCSB PDB site
Description: E-DreI
Class: Hydrolase/DNA
Keywords: LAGLIDADG, homing, engineering, design, endonuclease, structure
Deposited on 2002-09-10, released 2002-11-29
The last revision prior to the SCOP 1.73 freeze date was dated 2002-11-29, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.231
AEROSPACI score: -1.71 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: chimera of homing endonuclease I-DmoI and DNA endonuclease I-CreI
    Species: Desulfurococcus mobilis and Chlamydomonas reinhardtii
    Database cross-references and differences (RAF-indexed):
    • Uniprot p21505
      • see remark 999 (18)
      • see remark 999 (50)
      • see remark 999 (13)
      • linker (102-104)
      • see remark 999 (107)
      • see remark 999 (112)
      • see remark 999 (138)
      • see remark 999 (146)
      • see remark 999 (192-193)
      • see remark 999 (206-207)
    • Uniprot p05725
    Domains in SCOP 1.73: d1mowa1, d1mowa2
  • Chain 'B':
    Compound: 5'-d(*cp*cp*ap*ap*ap*cp*tp*gp*tp*cp*tp*cp*ap*ap*gp*tp*tp*cp*cp*gp*gp*cp*g)-3'
  • Chain 'C':
    Compound: 5'-d(*cp*gp*cp*cp*gp*gp*ap*ap*cp*tp*tp*gp*ap*gp*ap*cp*ap*gp*tp*tp*tp*gp*g)-3'
  • Chain 'D':
    Compound: chimera of homing endonuclease I-DmoI and DNA endonuclease I-CreI
    Species: Desulfurococcus mobilis and Chlamydomonas reinhardtii
    Database cross-references and differences (RAF-indexed):
    • Uniprot p21505
      • see remark 999 (18)
      • see remark 999 (50)
      • see remark 999 (13)
      • linker (102-104)
      • see remark 999 (107)
      • see remark 999 (112)
      • see remark 999 (138)
      • see remark 999 (146)
      • see remark 999 (192-193)
      • see remark 999 (206-207)
    • Uniprot p05725
    Domains in SCOP 1.73: d1mowd1, d1mowd2
  • Chain 'E':
    Compound: 5'-d(*cp*cp*ap*ap*ap*cp*tp*gp*tp*cp*tp*cp*ap*ap*gp*tp*tp*cp*cp*gp*gp*cp*g)-3'
  • Chain 'F':
    Compound: 5'-d(*cp*gp*cp*cp*gp*gp*ap*ap*cp*tp*tp*gp*ap*gp*ap*cp*ap*gp*tp*tp*tp*gp*g)-3'
  • Chain 'G':
    Compound: chimera of homing endonuclease I-DmoI and DNA endonuclease I-CreI
    Species: Desulfurococcus mobilis and Chlamydomonas reinhardtii
    Database cross-references and differences (RAF-indexed):
    • Uniprot p21505
      • see remark 999 (18)
      • see remark 999 (50)
      • see remark 999 (13)
      • linker (102-104)
      • see remark 999 (107)
      • see remark 999 (112)
      • see remark 999 (138)
      • see remark 999 (146)
      • see remark 999 (192-193)
      • see remark 999 (206)
    • Uniprot p05725
    Domains in SCOP 1.73: d1mowg1, d1mowg2
  • Chain 'H':
    Compound: 5'-d(*cp*cp*ap*ap*ap*cp*tp*gp*tp*cp*tp*cp*ap*ap*gp*tp*tp*cp*cp*gp*gp*cp*g)-3'
  • Chain 'I':
    Compound: 5'-d(*cp*gp*cp*cp*gp*gp*ap*ap*cp*tp*tp*gp*ap*gp*ap*cp*ap*gp*tp*tp*tp*gp*g)-3'
  • Chain 'J':
    Compound: chimera of homing endonuclease I-DmoI and DNA endonuclease I-CreI
    Species: Desulfurococcus mobilis and Chlamydomonas reinhardtii
    Database cross-references and differences (RAF-indexed):
    • Uniprot p21505 (Start-101)
      • see remark 999 (18)
      • see remark 999 (50)
      • see remark 999 (13)
      • linker (102-104)
      • see remark 999 (107)
      • see remark 999 (112)
      • see remark 999 (138)
      • see remark 999 (192-193)
      • see remark 999 (206-207)
    • Uniprot p05725 (105-End)
    Domains in SCOP 1.73: d1mowj1, d1mowj2
  • Chain 'K':
    Compound: 5'-d(*cp*cp*ap*ap*ap*cp*tp*gp*tp*cp*tp*cp*ap*ap*gp*tp*tp*cp*cp*gp*gp*cp*g)-3'
  • Chain 'L':
    Compound: 5'-d(*cp*gp*cp*cp*gp*gp*ap*ap*cp*tp*tp*gp*ap*gp*ap*cp*ap*gp*tp*tp*tp*gp*g)-3'
  • Heterogens: MG, SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1mowA (A:)
mhnnenvsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqfli
delnvkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsi
iaqikpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikp
lhnfltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkt
tsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1mowA (A:)
envsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqflideln
vkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsiiaqi
kpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikplhnf
ltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkttset
vravldsl

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1mowD (D:)
mhnnenvsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqfli
delnvkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsi
iaqikpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikp
lhnfltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkt
tsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1mowD (D:)
envsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqflideln
vkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsiiaqi
kpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikplhnf
ltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkttset
vravldsl

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1mowG (G:)
mhnnenvsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqfli
delnvkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsi
iaqikpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikp
lhnfltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkt
tsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1mowG (G:)
vsgisayllgliwgdgglyklnrseyrvvitqksenlikqfiaprmqflidelnvkskiq
ivkgdtryelrvsskklyyyfanmlerilfngnrflaylagivdgdgsiiaqikpnqsyk
fkhqlsltfqvtqkterrwfllvdeigvgyvrdrvsdyilseikplhnfltqlqpflnfk
qkqanlvlkiielpsakspdkflevctwvdqiaalndsktrkttsetvravld

  • Chain 'H':
    No sequence available.

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >1mowJ (J:)
mhnnenvsgisayllgliwgdgglyklkykgnrseyrvvitqksenlikqfiaprmqfli
delnvkskiqivkgdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsi
iaqikpnqsykfkhqlsltfqvtqkterrwfldklvdeigvgyvrdrgsvsdyilseikp
lhnfltqlqpflnfkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsktrkt
tsetvravldslsekkkssp

    Sequence, based on observed residues (ATOM records): (download) 
    >1mowJ (J:)
nvsgisayllgliwgdgglyklkynrseyrvvtqksenlikqfiaprmqflidelnvski
qivkdtryelrvsskklyyyfanmlerirlfngnrflaylagivdgdgsiiaqikpnqsy
kfkhqlsltfqvtqkrrwfvdeigvgyvrdrgsvsdyilseikplhnfltqlqpflnfkq
kqanlvlkiieqlpakespdkflevctwvdqiaalndsktrttsetvravldsl

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.