PDB entry 1mep

View 1mep on RCSB PDB site
Description: Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.
Class: Biotin-binding protein
Keywords: homotetramer, biotin-binding protein
Deposited on 2002-08-08, released 2003-09-02
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 1.65 Å
R-factor: 0.204
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Gene: core streptavidin
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (32)
      • engineered (115)
    Domains in SCOPe 2.08: d1mepa_
  • Chain 'B':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Gene: core streptavidin
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (32)
      • engineered (115)
    Domains in SCOPe 2.08: d1mepb_
  • Chain 'C':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Gene: core streptavidin
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (32)
      • engineered (115)
    Domains in SCOPe 2.08: d1mepc_
  • Chain 'D':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Gene: core streptavidin
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (32)
      • engineered (115)
    Domains in SCOPe 2.08: d1mepd_
  • Heterogens: BTN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1mepA (A:)
aeagitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1mepA (A:)
gitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftkvk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1mepB (B:)
aeagitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1mepB (B:)
gitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftkv

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1mepC (C:)
aeagitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1mepC (C:)
gitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftkv

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1mepD (D:)
aeagitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1mepD (D:)
gitgtwynqlgstfivtagadgaltgtyeaavgnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghatftkv