PDB entry 1lwt

View 1lwt on RCSB PDB site
Description: Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)
Class: hydrolase/DNA
Keywords: homing endonuclease, protein-DNA complex, intein, endonuclease
Deposited on 2002-06-03, released 2002-09-27
The last revision prior to the SCOP 1.73 freeze date was dated 2002-09-27, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 3.2 Å
R-factor: 0.242
AEROSPACI score: 0.13 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: endonuclease pi-scei
    Species: Saccharomyces cerevisiae
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (0-453)
      • modified residue (9)
      • modified residue (27)
      • modified residue (46)
      • modified residue (108)
      • modified residue (192)
      • modified residue (235)
      • modified residue (371)
      • modified residue (384)
    Domains in SCOP 1.73: d1lwta1, d1lwta2, d1lwta3
  • Chain 'B':
    Compound: PI-SceI DNA substrate top strand
  • Chain 'C':
    Compound: PI-SceI DNA substrate bottom strand
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1lwtA (A:)
cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
sdssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgriv
elvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtya
pilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervt
eyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgflkdgv
knipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglv
vsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfy
felqelkeddyygitlsddsdhqfllanqvvvhn

    Sequence, based on observed residues (ATOM records): (download) 
    >1lwtA (A:)
cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
ssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgrivel
vkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtyapi
lyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervtey
aeklnlcaeykdrkaktvnlyskvvrgngirnnlntenplwdaivglgflkdgvknipsf
lstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglvvsvnae
pakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfyfelqel
keddyygitlsddsdhqfllanqvvvhn

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.