PDB entry 1lwt

View 1lwt on RCSB PDB site
Description: Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)
Class: hydrolase/DNA
Keywords: homing endonuclease, protein-DNA complex, intein, endonuclease, hydrolase/DNA complex
Deposited on 2002-06-03, released 2002-09-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 3.2 Å
R-factor: 0.242
AEROSPACI score: 0.13 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: endonuclease pi-scei
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: VMA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P17255 (0-453)
      • modified residue (9)
      • modified residue (27)
      • modified residue (46)
      • modified residue (108)
      • modified residue (192)
      • modified residue (235)
      • modified residue (371)
      • modified residue (384)
    Domains in SCOPe 2.08: d1lwta1, d1lwta2, d1lwta3
  • Chain 'B':
    Compound: PI-SceI DNA substrate top strand
  • Chain 'C':
    Compound: PI-SceI DNA substrate bottom strand
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1lwtA (A:)
    cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
    sdssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgriv
    elvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtya
    pilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervt
    eyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgflkdgv
    knipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglv
    vsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfy
    felqelkeddyygitlsddsdhqfllanqvvvhn
    

    Sequence, based on observed residues (ATOM records): (download)
    >1lwtA (A:)
    cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhrahk
    ssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapdgrivel
    vkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtyapi
    lyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslmervtey
    aeklnlcaeykdrkaktvnlyskvvrgngirnnlntenplwdaivglgflkdgvknipsf
    lstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslarslglvvsvnae
    pakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfyfelqel
    keddyygitlsddsdhqfllanqvvvhn
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.