PDB entry 1kh5

View 1kh5 on RCSB PDB site
Description: e. coli alkaline phosphatase mutant (d330n) mimic of the transition states with aluminium fluoride
Class: hydrolase
Keywords: alkaline phosphatase, hydrolase
Deposited on 2001-11-29, released 2002-03-13
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-06-25, with a file datestamp of 2014-06-20.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.194
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alkaline phosphatase
    Species: Escherichia coli [TaxId:562]
    Gene: PHOAD153HD330N
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00634
      • conflict (14)
      • conflict (34)
      • conflict (175)
      • conflict (227)
      • conflict (229)
      • engineered (329)
    Domains in SCOPe 2.07: d1kh5a_
  • Chain 'B':
    Compound: alkaline phosphatase
    Species: Escherichia coli [TaxId:562]
    Gene: PHOAD153HD330N
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00634 (Start-448)
      • conflict (14)
      • conflict (34)
      • conflict (175)
      • conflict (227)
      • conflict (229)
      • engineered (329)
    Domains in SCOPe 2.07: d1kh5b_
  • Heterogens: ZN, MG, AF3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1kh5A (A:)
    tpempvlenraaqgnitapggarrltgdqtaalrnslsdkpakniilligdgmgdseita
    arnyaegaggffkgidalpltgqythyalnkktgkpdyvtdsaasatawstgvktyngal
    gvdihekdhptilemakaaglatgnvstaelqdatpaalvahvtsrkcygpsatsqkcpg
    nalekggkgsiteqllnaradvtlgggaktfaetatagewqgktlreeaeargyqlvsda
    aslnsvteanqqkpllglfadgnmpvrwlgpkatyhgnidkpavtctpnpqrndsvptla
    qmtdkaiellsknekgfflqvegasidkqnhaanpcgqigetvdldeavqralefakkeg
    ntlvivtadhahasqivapdtkapgltqalntkdgavmvmsygnseedsqehtgsqlria
    aygphaanvvgltdqtdlfytmkaalglk
    

    Sequence, based on observed residues (ATOM records): (download)
    >1kh5A (A:)
    vlenraaqgnitapggarrltgdqtaalrnslsdkpakniilligdgmgdseitaarnya
    egaggffkgidalpltgqythyalnkktgkpdyvtdsaasatawstgvktyngalgvdih
    ekdhptilemakaaglatgnvstaelqdatpaalvahvtsrkcygpsatsqkcpgnalek
    ggkgsiteqllnaradvtlgggaktfaetatagewqgktlreeaeargyqlvsdaaslns
    vteanqqkpllglfadgnmpvrwlgpkatyhgnidkpavtctpnpqrndsvptlaqmtdk
    aiellsknekgfflqvegasidkqnhaanpcgqigetvdldeavqralefakkegntlvi
    vtadhahasqivapdtkapgltqalntkdgavmvmsygnseedsqehtgsqlriaaygph
    aanvvgltdqtdlfytmkaalglk
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1kh5B (B:)
    tpempvlenraaqgnitapggarrltgdqtaalrnslsdkpakniilligdgmgdseita
    arnyaegaggffkgidalpltgqythyalnkktgkpdyvtdsaasatawstgvktyngal
    gvdihekdhptilemakaaglatgnvstaelqdatpaalvahvtsrkcygpsatsqkcpg
    nalekggkgsiteqllnaradvtlgggaktfaetatagewqgktlreeaeargyqlvsda
    aslnsvteanqqkpllglfadgnmpvrwlgpkatyhgnidkpavtctpnpqrndsvptla
    qmtdkaiellsknekgfflqvegasidkqnhaanpcgqigetvdldeavqralefakkeg
    ntlvivtadhahasqivapdtkapgltqalntkdgavmvmsygnseedsqehtgsqlria
    aygphaanvvgltdqtdlfytmkaalglk
    

    Sequence, based on observed residues (ATOM records): (download)
    >1kh5B (B:)
    vlenraaqgnitapggarrltgdqtaalrnslsdkpakniilligdgmgdseitaarnya
    egaggffkgidalpltgqythyalnkktgkpdyvtdsaasatawstgvktyngalgvdih
    ekdhptilemakaaglatgnvstaelqdatpaalvahvtsrkcygpsatsqkcpgnalek
    ggkgsiteqllnaradvtlgggaktfaetatagewqgktlreeaeargyqlvsdaaslns
    vteanqqkpllglfadgnmpvrwlgpkatyhgnidkpavtctpnpqrndsvptlaqmtdk
    aiellsknekgfflqvegasidkqnhaanpcgqigetvdldeavqralefakkegntlvi
    vtadhahasqivapdtkapgltqalntkdgavmvmsygnseedsqehtgsqlriaaygph
    aanvvgltdqtdlfytmkaalglk