PDB entry 1kfl

View 1kfl on RCSB PDB site
Description: Crystal structure of phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase) from E.coli complexed with Mn2+, PEP, and Phe
Class: lyase
Keywords: beta/alpha barrel, allosteric inhibition, feedback regulation, aromatic biosynthetic pathway
Deposited on 2001-11-21, released 2002-08-23
The last revision prior to the SCOP 1.73 freeze date was dated 2002-08-23, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.218
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kfla_
  • Chain 'B':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kflb_
  • Chain 'C':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kflc_
  • Chain 'D':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kfld_
  • Chain 'E':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kfle_
  • Chain 'F':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kflf_
  • Chain 'G':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kflg_
  • Chain 'H':
    Compound: 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase
    Species: Escherichia coli
    Gene: aroG
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00886 (0-349)
      • cloning artifact (0)
      • cloning artifact (90)
      • cloning artifact (112)
      • cloning artifact (146)
      • cloning artifact (156)
      • cloning artifact (262)
      • cloning artifact (278)
      • cloning artifact (299)
    Domains in SCOP 1.73: d1kflh_
  • Heterogens: MN, SO4, PHE, PEP

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflA (A:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflB (B:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflC (C:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflD (D:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflE (E:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflF (F:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflG (G:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1kflH (H:)
mnyqnddlrikeikellppvallekfpatenaantvaharkaihkilkgnddrllvvigp
csihdpvaakeyatrllalreelkdeleivmrvyfekprttvgwkglindphmdnsfqin
dglriarkllldindsglpaagefldmitpqyladlmswgaigarttesqvhrelasgls
cpvgfkngtdgtikvaidainaagaphcflsvtkwghsaivntsgngdchiilrggkepn
ysakhvaevkeglnkaglpaqvmidfshansskqfkkqmdvcadvcqqiaggekaiigvm
veshlvegnqslesgeplaygksitdacigwedtdallrqlanavkarrg