PDB entry 1k83

View 1k83 on RCSB PDB site
Description: Crystal Structure of Yeast RNA Polymerase II Complexed with the Inhibitor Alpha Amanitin
Class: transcription/toxin
Keywords: transcription-toxin complex, alpha amanitin, toxin, inhibitor, polymerase, transferase, DNA binding, zinc-finger, phosphoprotein, transcription, ubl transcription-toxin complex
Deposited on 2001-10-22, released 2002-02-13
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-07-27, with a file datestamp of 2011-07-22.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.229
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase II largest subunit
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83a_
  • Chain 'B':
    Compound: DNA-directed RNA polymerase II 140kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83b_
  • Chain 'C':
    Compound: DNA-directed RNA polymerase II 45kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83c1, d1k83c2
  • Chain 'E':
    Compound: DNA-directed RNA polymerase II 27kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83e1, d1k83e2
  • Chain 'F':
    Compound: DNA-directed RNA polymerase II 23kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83f_
  • Chain 'H':
    Compound: DNA-directed RNA polymerase II 14.5kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83h_
  • Chain 'I':
    Compound: DNA-directed RNA polymerase II 14.2kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83i1, d1k83i2
  • Chain 'J':
    Compound: DNA-directed RNA polymerase II 8.3kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83j_
  • Chain 'K':
    Compound: DNA-directed RNA polymerase II 13.6kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83k_
  • Chain 'L':
    Compound: DNA-directed RNA polymerase II 7.7kd polypeptide
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene:
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d1k83l_
  • Chain 'M':
    Compound: alpha amanitin
    Species: Amanita phalloides [TaxId:67723]
    Gene:
    Database cross-references and differences (RAF-indexed):
  • Heterogens: ZN, MN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1k83A (A:)
    mvgqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgs
    idrnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehne
    lmrqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglk
    lvgswkkdratgdadepelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclp
    vppppvrpsisfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhv
    atymdndiagqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnle
    ldqvgvpksiaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskr
    agdiqlqygwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspyna
    dfdgdemnlhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtf
    ieldqvlnmlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllsp
    kdngmliidgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhn
    gfstgigdtiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvr
    flneardkagrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfv
    drtlphfskddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrr
    lvkaledimvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvd
    llntdhtldpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvni
    rriiqnaqqtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfcc
    llrsrlatrrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmt
    lntfhfagvaskkvtsgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaieh
    ttlksvtiaseiyydpdprstvipedeeiiqlhfslldeeaeqsfdqqspwllrleldra
    amndkdltmgqvgerikqtfkndlfviwsedndekliircrvvrpksldaeteaeedhml
    kkientmlenitlrgveniervvmmkydrkvpsptgeyvkepewvletdgvnlsevmtvp
    gidptriytnsfidimevlgieagraalykevynviasdgsyvnyrhmallvdvmttqgg
    ltsvtrhgfnrsntgalmrcsfeetveilfeagasaelddcrgvsenvilgqmapigtga
    fdvmideeslvkympeqkiteiedgqdggvtpysnesglvnadldvkdelmfsplvdsgs
    ndamaggftayggadygeatspfgaygeaptspgfgvsspgfsptsptysptspayspts
    psysptspsysptspsysptspsysptspsysptspsysptspsysptspsysptspsys
    ptspsysptspsysptspsysptspsysptspsysptspaysptspsysptspsysptsp
    sysptspsysptspnysptspsysptspgyspgspayspkqdeqkhnenensr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83A (A:)
    qyssaplrtvkevqfglfspeevraisvakirfpekigglndprlgsidrnlkcqtcqeg
    mnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehnelmrqalaikdskk
    rfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglklvgswkkepelrv
    lsteeilnifkhisvkdftslgfnevfsrpewmiltclpvppppvrddltfkladilkan
    isletlehngaphhaieeaesllqfhvatymdndiagqsirarlkgkegrivdfsartvi
    sgdpnleldqvgvpksiaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdri
    dlryskragdiqlqygwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnls
    vtspynadfdgdemnlhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirk
    ltlrdtfieldqvlnmlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfde
    gttllspkdngmliidgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvv
    nfwllhngfstgigdtiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlres
    fednvvrflneardkagrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegk
    riafgfvdrtlphfskddyspeskgfvensylrgltpqefffhamggreglidtavktae
    tgyiqrrlvkaledimvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaaf
    ekryrvdllntdhtldpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgean
    wplpvnirriiqnaqqtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrd
    avtlfccllrsrlatrrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsig
    epatqmtlkkvtsgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaiehttl
    ksvtiaseiyydpdprstvipedeeiiqlhfsqqspwllrleldraamndkdltmgqvge
    rikqtfkndlfviwsedndekliircrvvaeedhmlkkientmlenitlrgveniervvm
    mkydrkvpsptgeyvkepewvletdgvnlsevmtvpgidptriytnsfidimevlgieag
    raalykevynviasdgsyvnyrhmallvdvmttqggltsvtrhgfnrsntgalmrcsfee
    tveilfeagasaelddcrgvsenvilgqmapigtgafdvmideesl
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1k83B (B:)
    msdlansekyydedpygfedesapitaedswavisaffrekglvsqqldsfnqfvdytlq
    diicedstlileqlaqhttesdnisrkyeisfgkiyvtkpmvnesdgvthalypqearlr
    nltyssglfvdvkkrtyeaidvpgrelkyeliaeeseddsesgkvfigrlpimlrskncy
    lseatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaei
    rsalekgsrfistlqvklygregssartikatlpyikqdipiviifralgiipdgeileh
    icydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkef
    lphitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktl
    fkkltkdifrymqrtveeahdfnmklainaktitsglkyalatgnwgeqkkamssragvs
    qvlnrytysstlshlrrtntpigrdgklakprqlhnthwglvcpaetpegqacglvknls
    lmscisvgtdpmpiitflsewgmepledyvphqspdatrvfvngvwhgvhrnparlmetl
    rtlrrkgdinpevsmirdirekelkiftdagrvyrplfiveddeslghkelkvrkghiak
    lmateyqdieggfedveeytwssllneglveyidaeeeesiliamqpedlepaeaneend
    ldvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprntyqsamgkqamgv
    fltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaiacysgynqedsmi
    mnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtydkldddglia
    pgvrvsgedviigkttpispdeeelgqrtayhskrdastplrstengivdqvlvttnqdg
    lkfvkvrvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsr
    mtvahliecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkk
    lmaqiffgptyyqrlrhmvddkiharargpmqvltrqpvegrsrdgglrfgemerdcmia
    hgaasflkerlmeasdafrvhicgicglmtviaklnhnqfeckgcdnkidiyqihipyaa
    kllfqelmamnitprlytdrsrdf
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83B (B:)
    fedesapitaedswavisaffrekglvsqqldsfnqfvdytlqdiicedstlieisfgki
    yvtkpmvnesdgvthalypqearlrnltyssglfvdvkkrtykvfigrlpimlrskncyl
    seatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaeir
    salekgsrfistlqvklygregssartikatlpyikqdipiviifralgiipdgeilehi
    cydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkefl
    phitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktlf
    kkltkdifrlainaktitsglkyalatgnwgvsqvlnrytysstlshlrrtntpiakprq
    lhnthwglvcpaetpegqacglvknlslmscisvgtdpmpiitflsewgmepledyvphq
    spdatrvfvngvwhgvhrnparlmetlrtlrrkgdinpevsmirdirekelkiftdagrv
    yrplfiveddeslghkelkvrkghiaklmateyqdeytwssllneglveyidaeeeesil
    iamqpedlepdvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprntyqs
    amgkqamgvfltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaiacys
    gynqedsmimnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtyd
    kldddgliapgvrvsgedviigkttpskrdastplrstengivdqvlvttnqdglkfvkv
    rvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsrmtvahl
    iecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkklmaqif
    fgptyyqrlrhmvddkiharargpglrfgemerdcmiahgaasflkerlmeasdafrvhi
    cgicglmtviaklnhnqfeckgcdnkidiyqihipyaakllfqelmamnitprlytdrsr
    df
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >1k83C (C:)
    mseegpqvkireaskdnvdfilsnvdlamanslrrvmiaeiptlaidsvevetnttvlad
    efiahrlgliplqsmdieqleysrdcfcedhcdkcsvvltlqafgesesttnvyskdlvi
    vsnlmgrnighpiiqdkegngvlicklrkgqelkltcvakkgiakehakwgpaaaiefey
    dpwnklkhtdywyeqdsakewpqsknceyedppnegdpfdykaqadtfymnvesvgsipv
    dqvvvrgidtlqkkvasillaltqmdqdkvnfasgdnntasnmlgsnedvmmtgaeqdpy
    snasqmgntgsggydnaw
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83C (C:)
    eegpqvkireaskdnvdfilsnvdlamanslrrvmiaeiptlaidsvevetnttvladef
    iahrlgliplqsmdieqleysrdcfcedhcdkcsvvltlqafgesesttnvyskdlvivs
    nlmgrnighpiiqdkegngvlicklrkgqelkltcvakkgiakehakwgpaaaiefeydp
    wnklkhtdywyeqdsakewpqsknceyedppnegdpfdykaqadtfymnvesvgsipvdq
    vvvrgidtlqkkvasillaltqmdqd
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >1k83E (E:)
    mdqenernisrlwrafrtvkemvkdrgyfitqeevelpledfkakycdsmgrpqrkmmsf
    qanpteesiskfpdmgslwvefcdepsvgvktmktfvihiqeknfqtgifvyqnnitpsa
    mklvpsippatietfneaalvvnithhelvpkhirlssdekrellkryrlkesqlpriqr
    adpvalylglkrgevvkiirksetsgryasyricm
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83E (E:)
    qenernisrlwrafrtvkemvkdrgyfitqeevelpledfkakycdsmgrpqrkmmsfqa
    npteesiskfpdmgslwvefcdepsvgvktmktfvihiqeknfqtgifvyqnnitpsamk
    lvpsippatietfneaalvvnithhelvpkhirlssdekrellkryrlkesqlpriqrad
    pvalylglkrgevvkiirksetsgryasyricm
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >1k83F (F:)
    msdyeeafndgnenfedfdvehfsdeetyeekpqfkdgettdangktivtggngpedfqq
    heqirrktlkekaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplri
    amkelaekkiplvirrylpdgsfedwsveelivdl
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83F (F:)
    kaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplriamkelaekkip
    lvirrylpdgsfedwsveelivdl
    

  • Chain 'H':
    Sequence, based on SEQRES records: (download)
    >1k83H (H:)
    msntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtia
    sslnledtpandssatrswrppqagdrsladdydyvmygtaykfeevskdliavyysfgg
    llmrlegnyrnlnnlkqenayllirr
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83H (H:)
    sntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtias
    sltrswrppqagdrsladdydyvmygtaykfeevskdliavyysfggllmrlegnyrnln
    nlkqenayllirr
    

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records: (download)
    >1k83I (I:)
    mttfrfcrdcnnmlypredkennrllfecrtcsyveeagsplvyrhelitnigetagvvq
    digsdptlprsdrecpkchsrenvffqsqqrrkdtsmvlffvclscshiftsdqknkrtq
    fs
    

  • Chain 'J':
    Sequence, based on SEQRES records: (download)
    >1k83J (J:)
    mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
    lrynplekrd
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83J (J:)
    mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
    lrynp
    

  • Chain 'K':
    Sequence, based on SEQRES records: (download)
    >1k83K (K:)
    mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
    ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtlaaddaf
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83K (K:)
    mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
    ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtl
    

  • Chain 'L':
    Sequence, based on SEQRES records: (download)
    >1k83L (L:)
    msregfqiptnldaaaagtsqartatlkyicaecssklslsrtdavrckdcghrillkar
    tkrlvqfear
    

    Sequence, based on observed residues (ATOM records): (download)
    >1k83L (L:)
    tlkyicaecssklslsrtdavrckdcghrillkartkrlvqfear
    

  • Chain 'M':
    No sequence available.