PDB entry 1hjg

View 1hjg on RCSB PDB site
Description: alteration of the co-substrate selectivity of deacetoxycephalosporin c synthase: the role of arginine-258
Class: oxidoreductase
Keywords: alternative 2-oxoacids, cephem antibiotic biosynthesis, chemical cosubstrate rescue, co-substrate selectivity, 2-oxoglutarate-dependent oxygenase
Deposited on 2001-01-15, released 2001-06-01
The last revision prior to the SCOP 1.73 freeze date was dated 2003-04-01, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: 0.225
AEROSPACI score: 0.58 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: deacetoxycephalosporin c synthase
    Species: Streptomyces clavuligerus
    Gene: CEFE, R258Q MUTANT
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18548 (0-End)
      • engineered mutation (257)
      • see remark 999 (49)
    Domains in SCOP 1.73: d1hjga_
  • Heterogens: FE2, KIV, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1hjgA (A:)
mdttvptfslaelqqglhqdefrrclrdkglfyltdcgltdtelksakdividffehgse
aekravtspvptmrrgftglesestaqitntgsysdysmcysmgtadnlfpsgdferiwt
qyfdrqytasravarevlratgtepdggveafldcepllrfryfpqvpehrsaeeqplrm
aphydlsmvtliqqtpcangfvslqaevggaftdlpyrpdavlvfcgaiatlvtggqvka
prhhvaaprrdqiagssqtssvfflrpnadftfsvplarecgfdvsldgetatfqdwigg
nyvnirrtska

    Sequence, based on observed residues (ATOM records): (download) 
    >1hjgA (A:)
mdttvptfslaelqqglhqdefrrclrdkglfyltdcgltdtelksakdividffehgse
aekravtspvptmrrgftglsmcysmgtadnlfpsgdferiwtqyfdrqytasravarev
lratgtepdggveafldcepllrfryfplrmaphydlsmvtliqqtfvslqaevggaftd
lpyrpdavlvfcgaiatlvtggqvkaprhhvaasqtssvfflrpnadftfsvplarecgf
dvsldgetatfqdwiggnyvnirr