PDB entry 1hjf

View 1hjf on RCSB PDB site
Description: Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258
Class: oxidoreductase
Keywords: oxidoreductase, alternative 2-oxoacids, cephem antibiotic biosynthesis, chemical cosubstrate rescue, co-substrate selectivity, 2- oxoglutarate-dependent oxygenase
Deposited on 2001-01-15, released 2001-06-01
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-05-08, with a file datestamp of 2019-05-03.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: deacetoxycephalosporin c synthase
    Species: Streptomyces clavuligerus [TaxId:1901]
    Gene: CEFE, R258Q MUTANT
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18548 (0-End)
      • engineered mutation (257)
      • see remark 999 (49)
    Domains in SCOPe 2.08: d1hjfa_
  • Heterogens: FE2, COI, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1hjfA (A:)
mdttvptfslaelqqglhqdefrrclrdkglfyltdcgltdtelksakdividffehgse
aekravtspvptmrrgftglesestaqitntgsysdysmcysmgtadnlfpsgdferiwt
qyfdrqytasravarevlratgtepdggveafldcepllrfryfpqvpehrsaeeqplrm
aphydlsmvtliqqtpcangfvslqaevggaftdlpyrpdavlvfcgaiatlvtggqvka
prhhvaaprrdqiagssqtssvfflrpnadftfsvplarecgfdvsldgetatfqdwigg
nyvnirrtska

    Sequence, based on observed residues (ATOM records): (download) 
    >1hjfA (A:)
mdttvptfslaelqqglhqdefrrclrdkglfyltdcgltdtelksakdividffehgse
aekravtspvptmrrgftglestgsysdysmcysmgtadnlfpsgdferiwtqyfdrqyt
asravarevlratgtepdggveafldcepllrfryfplrmaphydlsmvtliqqtpfvsl
qaevggaftdlpyrpdavlvfcgaiatlvtggqvkaprhhvaapsqtssvfflrpnadft
fsvplarecgfdvsldgetatfqdwiggnyvnirrtsk