PDB entry 1gkl

View 1gkl on RCSB PDB site
Description: S954A mutant of the feruloyl esterase module from clostridium thermocellum complexed with ferulic acid
Class: hydrolase
Keywords: hydrolase, esterase family 1, inactive mutant
Deposited on 2001-08-15, released 2001-12-13
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-10-09, with a file datestamp of 2019-10-04.
Experiment type: XRAY
Resolution: 1.4 Å
R-factor: N/A
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: endo-1,4-beta-xylanase y
    Species: Clostridium thermocellum [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 1GKL (289-296)
      • conflict (228-229)
      • engineered mutation (165)
    • Uniprot P51584 (Start-288)
      • conflict (228-229)
      • engineered mutation (165)
    Domains in SCOPe 2.08: d1gkla1, d1gkla2
  • Chain 'B':
    Compound: endo-1,4-beta-xylanase y
    Species: Clostridium thermocellum [TaxId:1515]
    Database cross-references and differences (RAF-indexed):
    • PDB 1GKL (289-296)
      • conflict (228-229)
      • engineered mutation (165)
    • Uniprot P51584 (Start-288)
      • conflict (228-229)
      • engineered mutation (165)
    Domains in SCOPe 2.08: d1gklb1, d1gklb2
  • Heterogens: FER, GOL, ACT, CD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1gklA (A:)
masdkfpvaenpsssfkyesavqyrpapdsylnpcpqagrivketytgingtkslnvylp
ygydpnkkynifylmhgggenentifsndvklqnildhaimngeleplivvtptfnggnc
taqnfyqefrqnvipfveskystyaesttpqgiaasrmhrgfggfamgglttwyvmvncl
dyvayfmplsgdywygnspqdkansiaeainrsglskreyfvfaatgsediayanmnpqi
eamkalphfdytsdfskgnfyflvapgathwwgyvrhyiydalpyffhelehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1gklA (A:)
sfkyesavqyrpapdsylnpcpqagrivketytgingtkslnvylpygydpnkkynifyl
mhgggenentifsndvklqnildhaimngeleplivvtptfnggnctaqnfyqefrqnvi
pfveskystyaesttpqgiaasrmhrgfggfamgglttwyvmvncldyvayfmplsgdyw
ygnspqdkansiaeainrsglskreyfvfaatgsediayanmnpqieamkalphfdytsd
fskgnfyflvapgathwwgyvrhyiydalpyffhelehhhhhh

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1gklB (B:)
masdkfpvaenpsssfkyesavqyrpapdsylnpcpqagrivketytgingtkslnvylp
ygydpnkkynifylmhgggenentifsndvklqnildhaimngeleplivvtptfnggnc
taqnfyqefrqnvipfveskystyaesttpqgiaasrmhrgfggfamgglttwyvmvncl
dyvayfmplsgdywygnspqdkansiaeainrsglskreyfvfaatgsediayanmnpqi
eamkalphfdytsdfskgnfyflvapgathwwgyvrhyiydalpyffhelehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >1gklB (B:)
sfkyesavqyrpapdsylnpcpqagrivketytgingtkslnvylpygydpnkkynifyl
mhgggenentifsndvklqnildhaimngeleplivvtptfnggnctaqnfyqefrqnvi
pfveskystyaesttpqgiaasrmhrgfggfamgglttwyvmvncldyvayfmplsgdyw
ygnspqdkansiaeainrsglskreyfvfaatgsediayanmnpqieamkalphfdytsd
fskgnfyflvapgathwwgyvrhyiydalpyffhelehhhhhh