PDB entry 1gkf

View 1gkf on RCSB PDB site
Description: Crystal structures of penicillin acylase enzyme-substrate complexes: Structural insights into the catalytic mechanism
Class: hydrolase
Keywords: antibiotic resistance, hydrolase
Deposited on 2001-08-13, released 2002-01-04
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-07-24, with a file datestamp of 2019-07-19.
Experiment type: XRAY
Resolution: 1.41 Å
R-factor: N/A
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: penicillin g acylase alpha subunit
    Species: Escherichia coli [TaxId:562]
    Gene: PAC
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06875
      • modified residue (15)
    Domains in SCOPe 2.08: d1gkf.1
  • Chain 'B':
    Compound: penicillin g acylase beta subunit
    Species: Escherichia coli [TaxId:562]
    Gene: PAC
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06875 (0-556)
      • engineered mutation (240)
    Domains in SCOPe 2.08: d1gkf.1
  • Heterogens: EDO, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1gkfA (A:)
eqssseikivrdeygmphiyandtwhlfygygyvvaqdrlfqmemarrstqgtvaevlgk
dfvkfdkdirrnywpdairaqiaalspedmsilqgyadgmnawidkvntnpetllpkqfn
tfgftpkrwepfdvamifvgtmanrfsdstseidnlalltalkdkygvsqgmavfnqlkw
lvnpsapttiavqesnyplkfnqqnsqtaallprydlpapmldrpakgadgallalaagk
nretiaaqfaqgganglagy

    Sequence, based on observed residues (ATOM records): (download) 
    >1gkfA (A:)
ssseikivrdeygmphiyandtwhlfygygyvvaqdrlfqmemarrstqgtvaevlgkdf
vkfdkdirrnywpdairaqiaalspedmsilqgyadgmnawidkvntnpetllpkqfntf
gftpkrwepfdvamifvgtmanrfsdstseidnlalltalkdkygvsqgmavfnqlkwlv
npsapttiavqesnyplkfnqqnsqtaa

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1gkfB (B:)
snmwvigkskaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvfghn
gviswgstagfgddvdifaerlsaekpgyylhngkwvkmlsreetitvkngqaetftvwr
tvhgnilqtdqttqtayaksrawdgkevasllawthqmkaknwqewtqqaakqaltinwy
yadvngnigyvhtgaypdrqsghdprlpvpgtgkwdwkgllpfemnpkvynpqsgyianw
anspqkdypasdlfaflwggadrvteidrlleqkprltadqawdvirqtsrqdlnlrlfl
ptlqaatsgltqsdprrqlvetltrwdginllnddgktwqqpgsailnvwltsmlkrtvv
aavpmpfdkwysasgyettqdgptgslnisvgakilyeavqgdkspipqavdlfagkpqq
evvlaaledtwetlskrygnnvsnwktpamaltfrannffgvpqaaaeetrhqaeyqnrg
tendmivfspttsdrpvlawdvvapgqsgfiapdgtvdkhyedqlkmyenfgrkslwltk
qdveahkesqevlhvqr