PDB entry 1ef0

View 1ef0 on RCSB PDB site
Description: crystal structure of pi-scei miniprecursor
Class: hydrolase
Keywords: endonuclease, protein splicing, mini-precursor
Deposited on 2000-02-04, released 2000-06-07
The last revision prior to the SCOP 1.73 freeze date was dated 2003-04-01, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.231
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pi-scei endonuclease
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed):
    • GB AAB63978 (4-458)
      • see remark 999 (2-3)
      • engineered (4)
      • engineered (457)
    Domains in SCOP 1.73: d1ef0a1, d1ef0a2, d1ef0a3
  • Chain 'B':
    Compound: pi-scei endonuclease
    Species: Saccharomyces cerevisiae
    Database cross-references and differences (RAF-indexed):
    • GB AAB63978 (4-458)
      • see remark 999 (0-3)
      • engineered (4)
      • engineered (457)
      • see remark 999 (459-461)
    Domains in SCOP 1.73: d1ef0b1, d1ef0b2, d1ef0b3
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1ef0A (A:)
klegafakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqh
rahksdssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapd
grivelvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkaty
qtyapilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslm
ervteyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgfl
kdgvknipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslars
lglvvsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarec
rgfyfelqelkeddyygitlsddsdhqfllanqvvvhacger

    Sequence, based on observed residues (ATOM records): (download) 
    >1ef0A (A:)
egafakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqhra
hksdssrevpellkftcnathelvvrtprsvrvitfemgqkkapdgrivelvkevsksyp
isegperanelvesyrkasnkayfewtieardlsllgshvrkatyqtyapilyendhffd
ymqksktiegpkvlayllglwigdglsdratfsvdsrdtslmervteyaeklnlcaeykd
rkepqvaktvnlysenplwdaivglgflkdgvknipsflstdnigtretflaglidsdgy
vtdehgikatiktihtsvrdglvslarslglvvsvnaepakvdgtkhkisyaiymsggdv
llnvlskcagskkfrpapaaafarecrgfyfelqelkeddyygitlsddsdhqfllanqv
vvhac

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1ef0B (B:)
klegafakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksqh
rahksdssrevpellkftcnathelvvrtprsvrrlsrtikgveyfevitfemgqkkapd
grivelvkevsksypisegperanelvesyrkasnkayfewtieardlsllgshvrkaty
qtyapilyendhffdymqkskfhltiegpkvlayllglwigdglsdratfsvdsrdtslm
ervteyaeklnlcaeykdrkepqvaktvnlyskvvrgngirnnlntenplwdaivglgfl
kdgvknipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdglvslars
lglvvsvnaepakvdmngtkhkisyaiymsggdvllnvlskcagskkfrpapaaafarec
rgfyfelqelkeddyygitlsddsdhqfllanqvvvhacger

    Sequence, based on observed residues (ATOM records): (download) 
    >1ef0B (B:)
klegafakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqkslk
ftcnathelvvrtprsvrrlsrveyfevitfemgqkkapdgrivelvkevsksypisegp
eranelvesyrkasnkayfewtieardlsllgshvrkatyqtyapilyendhffdymqkv
layllglwigdglsdratfsvdsrdtslmervteyaeklnlcaeykdrkepqvaktvnly
sglgflkdgvknipsflstdnigtretflaglidsdgyvtdehgikatiktihtsvrdgl
vslarslglvvsvnisyaiymsggdvllnvlskcagskkfrpapaaafarecrgfyfelq
elkeddyygitlsddsdhqfllanqvvvhacger