PDB entry 1e0t

View 1e0t on RCSB PDB site
Description: r292d mutant of e. coli pyruvate kinase
Class: phosphotransferase
Keywords: glycolysis, allostery
Deposited on 2000-04-10, released 2000-04-11
The last revision prior to the SCOP 1.75 freeze date was dated 2003-04-01, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.246
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • conflict (278)
      • engineered mutation (291)
    Domains in SCOP 1.75: d1e0ta1, d1e0ta2, d1e0ta3
  • Chain 'B':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • conflict (278)
      • engineered mutation (291)
    Domains in SCOP 1.75: d1e0tb1, d1e0tb2, d1e0tb3
  • Chain 'C':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • conflict (278)
      • engineered mutation (291)
    Domains in SCOP 1.75: d1e0tc1, d1e0tc2, d1e0tc3
  • Chain 'D':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • conflict (278)
      • engineered mutation (291)
    Domains in SCOP 1.75: d1e0td1, d1e0td2, d1e0td3
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1e0tA (A:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmmldsmiknprptdaeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0tA (A:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmrptdaeagdvanaildgtdav
mlsgepleavsimaticertdrvmnsrleiteavcrgavetaekldaplivvatqggksa
ravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddfyrlgkelalqsglah
kgdvvvmvsgalvpsgttntasvhvl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1e0tB (B:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmmldsmiknprptdaeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0tB (B:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmrptdaeagdvanaildgtdav
mlsgepleavsimaticertdrvmnsrleiteavcrgavetaekldaplivvatqggksa
ravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddfyrlgkelalqsglah
kgdvvvmvsgalvpsgttntasvhvl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1e0tC (C:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmmldsmiknprptdaeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0tC (C:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmrptdaeagdvanaildgtdav
mlsgepleavsimaticertdrvmnsrleiteavcrgavetaekldaplivvatqggksa
ravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddfyrlgkelalqsglah
kgdvvvmvsgalvpsgttntasvhvl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1e0tD (D:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmmldsmiknprptdaeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0tD (D:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekcirarkvvitatmrptdaeagdvanaildgtdav
mlsgepleavsimaticertdrvmnsrleiteavcrgavetaekldaplivvatqggksa
ravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddfyrlgkelalqsglah
kgdvvvmvsgalvpsgttntasvhvl