PDB entry 1b3s

View 1b3s on RCSB PDB site
Description: structural response to mutation at a protein-protein interface
Class: hydrolase/hydrolase inhibitor
Keywords: RNAse-inhibitor complex, interfacial double mutant, hydrolase/hydrolase inhibitor complex
Deposited on 1998-12-01, released 1998-12-09
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.39 Å
R-factor: 0.247
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (0-109)
      • engineered (101)
    Domains in SCOPe 2.08: d1b3sa_
  • Chain 'B':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (0-109)
      • engineered (101)
    Domains in SCOPe 2.08: d1b3sb_
  • Chain 'C':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-109)
      • engineered (101)
    Domains in SCOPe 2.08: d1b3sc_
  • Chain 'D':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (1-89)
      • see remark 999 (0)
      • engineered (29)
    Domains in SCOPe 2.08: d1b3sd_
  • Chain 'E':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (1-89)
      • engineered (29)
    Domains in SCOPe 2.08: d1b3se_
  • Chain 'F':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (1-89)
      • engineered (29)
    Domains in SCOPe 2.08: d1b3sf_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1b3sA (A:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkir

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1b3sB (B:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkir

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1b3sC (C:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkir

    Sequence, based on observed residues (ATOM records): (download) 
    >1b3sC (C:)
vintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnregk
lpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkir

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1b3sD (D:)
mkkavingeqirsisdlhqtlkkelalpefygenldalwdcltgwveyplvlewrqfeqs
kqltengaesvlqvfreakaegcditiils

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1b3sE (E:)
mkkavingeqirsisdlhqtlkkelalpefygenldalwdcltgwveyplvlewrqfeqs
kqltengaesvlqvfreakaegcditiils

    Sequence, based on observed residues (ATOM records): (download) 
    >1b3sE (E:)
kkavingeqirsisdlhqtlkkelalpefygenldalwdcltgwveyplvlewrqfgaes
vlqvfreakaegcditiils

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >1b3sF (F:)
mkkavingeqirsisdlhqtlkkelalpefygenldalwdcltgwveyplvlewrqfeqs
kqltengaesvlqvfreakaegcditiils

    Sequence, based on observed residues (ATOM records): (download) 
    >1b3sF (F:)
kkavingeqirsisdlhqtlkkelalpefygenldalwdcltgwveyplvlewrqfeqsk
qltengaesvlqvfreakaegcditiils